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- 重疊延伸PCR法overlap extension by polymerase chain reaction
- 方法設計引物,采用PCR法分別擴增UreB表位多肽編碼基因Uepi和LTB編碼基因,重疊延伸PCR法將兩段基因拼接,T-A克隆后,構建融合基因表達質(zhì)粒pET-22b(+)-Uepi-LTB,經(jīng)酶切鑒定后轉化E.Methods Amplify the genes encoding UreB epitope polypeptide and LTB by PCR respectively and link by overlap extension PCR. After T-A cloning,the linked gene Uepi-LTB was cloned into prokaryotic expression vector pET-22b(+),and the constructed recombinant plasmid pET-22b(+)-Uepi-LTB was transformed to E.
- 重疊延伸PCRoverlap extension PCR
- 重疊延伸PCR（SOEPCR）splicing by overlap extension PCR
- 拼接重疊延伸PCRSOE-PCR
- 除法division
- 經(jīng)重疊延伸PCR擴增，將vH和vL連接后構建的ScFv基因大約75obp;The weight of ScFv about 750bp after VH linked with VL by SOE PCR.
- 賓夕法尼亞Pennsylvania
- 重疊overlapping
- 表示法notation
- 比色法colorimetry
- 循環(huán)延伸PCRrecursive PCR
- 重疊延伸overlap extension
- 層次分析法analytic hierarchy process (AHP)
- 測定法mensuration
- 連續延伸PCR方法a successive PCR method
- 企業(yè)法law of enterprise
- 重疊的overlapped
- 知識產(chǎn)權法law of knowledge property
- 重疊延伸聚合酶鏈式反應overlap-PCR
