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- 結論制備的可溶性抗Fas?Fab抗體具有抗Fas抗體的特異性,為進(jìn)一步的研究奠定了基礎。Anti Fas Fab phage antibody displaying on phage surface and soluble anti Fas Fab showed specific binding activity to Fas by ELISA. Conclusion The successful preparation of anti Fas Fab antibody paved a way for further study and application.
- 抗Fas抗體Anti-Fas antibody
- 目的:研究抗Fas抗體對人腎小球系膜細胞的致凋亡作用,探討Fas?FasL在腎臟損傷中的作用。Objective:To investigate the mechanism inducing apptosis of human glomerular mesangial cells (Mcs) by anti Fas antibody.
- 抗Fas抗體誘導維甲酸分化后的髓母細胞瘤細胞凋亡All-trans retinoic acid sensitizes human medulloblastoma cells Med-3 to anti-Fas antibody mediated apoptosis
- 用重組人Fas抗原進(jìn)行4輪篩選,得到100%的富集,說(shuō)明Fas重組抗原富集了抗Fas?Fab噬菌體抗體,經(jīng)ELISA檢測均有抗Fas抗體的特異性。The phage displaying antibody fragments were subjected to four rounds of panning with recombinant Fas antigen at solid phase. The eluted phage was enriched for nearly 100 fold and the percent of recombinant clones increased from 27%25 to 100%25 after four rounds of panning.
- Fas抗體Anti-Fas antibody
- 抗Fas單抗anti-Fas monoclonal antibody
- 結論:EJ細胞系對Fas抗體觸發(fā)細胞凋亡的敏感性依賴(lài)于細胞表面Fas的表達水平。the sensitivity of EJ cell line to Fas mediated apoptosis is mainly dependent upon the expression of Fas.
- 拮抗antagonism
- 拮抗劑antagonist
- 抗Fas錘頭狀核酶阻抑小鼠急性粒-單核白血病細胞免疫逃逸的研究Depressing the Immune Escape of Acute Myelomonocytic Leukemia via an Anti-Fas Ribozyme
- 經(jīng)10-6mol/LATRA或9?cisRA分別預處理4天后,HL?60細胞Fas表達水平及對抗Fas抗體誘導凋亡的敏感性均顯著(zhù)提高。After incubation of HL-60 cells with 10~-6?mol/L ATRA or 9?cis RA for 4 days, both the Fas expression and AFIA were significantly enhanced.
- 目的 研究細胞表面Fas 表達水平與抗Fas 治療生物學(xué)效應之間的關(guān)系。Objective To investigate the relationship between expression level of Fas and anti Fas biological responsiveness.
- 目的:探討全反式維甲酸(ATRA)和9?順式維甲酸(9?cisRA)對HL?60細胞Fas表達水平及維甲酸對抗Fas抗體誘導的細胞凋亡敏感性的影響。Objective: To investigate the effect of all?trans retinoic acid (ATRA) and 9?cis retinoic acid (9?cis RA) on the expression of Fas and the sensitivity to anti?Fas induced apotosis in HL-60 cells.
- 方法小兒急淋白血病細胞分別與Bcl?2反義脫氧寡核苷酸、抗Fas單抗培養,或與兩者一起培養,再分別定量檢測其凋亡細胞。Methods The percentage of apoptotic cells was detected after childhood ALL cells were incubated with Bcl 2 ASON, anti Fas MoAb or with both of them, respectively.
- 集魚(yú)設備
- 在無(wú)FasL表達情況下 ,抗Fas單克隆抗體能誘導RMA細胞凋亡。Apoptosis could be observed without the expression of FasL when anti Fas antibody was added to RMA cells.
- 白癜風(fēng)患者血清中抗黑素細胞膜表面抗原自身抗體的檢測Detection of Auto Antibodies to Melanocyte Surface Antigen in Sera of Patients with Vitiligo
- 抗CD3抗體anti-CD3 antibody
- 抗Clq抗體anti-Clq antibody