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- 基因重疊延伸拼接PCRSOE-PCR
- 重疊延伸拼接法從基因組DNA中獲得Ers-MIH1基因的編碼區Cloning of the coding region fragment of the Ers-MIH1 gene from genomic DNA by SOE method
- 重疊延伸拼接法splicing overlap extension; SOE
- 方法擴增EGFP及TK并采用重疊延伸拼接(SOE)法連接EGFP和TK,將EGFP-TK融合基因亞克隆至pBABE-puro-lox的Sal I和Cla I位點(diǎn)之間,構建成新載體pBGTKlox。METHODS:EGFP and TK were amplified by polymerase chain reaction and fused by splicing overlap extension. The fusion gene EGFP-TK was then subcloned to the sites Sal I and Cla I of pBABE-puro-lox to construct pBTKlox.
- 拼接PCRsplices PCR
- 重疊延伸PCRoverlap extension PCR
- 齊_(31)、武_(202)兩個(gè)微效多基因矮稈系含有3對以上微效基因,它們與被研究的所有隱性單基因和微效多基因重疊效應矮稈系的致矮力都屬中等。The dwarf lines Qi_(31) and Wu_(202) as controlled by more than 3minor polygenes and having overlapping effects of both types of genes had intermediatedwarfing ability.
- 拼接split joint
- 重疊延伸PCR法overlap extension by polymerase chain reaction
- 重疊overlapping
- NS3基因也是從NS1基因相同的起始位點(diǎn)開(kāi)始編碼,編碼了86個(gè)氨基酸后,有一個(gè)長(cháng)達1859個(gè)核苷酸的內含子,之后與VP1基因重疊錯位編碼剩余的21個(gè)氨基酸。NS1 gene was encoded from ATG to TAA. It was composed of 1989 nucleotides, encoded 662 amino acids.
- 群山向西延伸。The mountains have a western trend.
- 基因代代相傳。Genes are transmitted from one generation to another.
- 重疊延伸overlap extension
- 大片陸地延伸到海里。Land masses extrude into the sea.
- 分離的基因型a segregant genotype.
- 這條小河向北延伸。The small stream verges to the north.
- PCR重疊延伸技術(shù)splicing by overlap extension
- 以辦公室為中心的友誼通常不會(huì )延伸進(jìn)入休閑活動(dòng)或學(xué)?;顒?dòng)中去。Friendships that are centered around the office often do not spill out into recreational or even school activities.
- C基因C gene