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- 人U6啟動(dòng)子human U6 promoter
- U6啟動(dòng)子U6 promoter
- 方法構建利用U6啟動(dòng)子轉錄功能性小干擾RNA(siRNA)的質(zhì)粒載體,對陽(yáng)性克隆進(jìn)行酶切和測序鑒定后,轉染人甲狀腺癌FRO細胞,利用RT-PCR和W eston blot法檢測hTERT基因的表達情況。Methods A vector containing U6 promoter used to transcribe functional small interfering RNA(siRNA) targeting hTRET gene was constructed. After identification by restriction enzyme digestion and sequencing,the plasmid of positive clones was transfected into human thyroid carcinoma FRO cell line. The expression of hTRET gene was detected by RT-PCR and Western blot.
- 子midnight
- 啟動(dòng)to start
- 方法:設計和構建由U6啟動(dòng)子驅動(dòng)產(chǎn)生hTERT的siRNA表達質(zhì)粒,轉染至ALVA-41前列腺腫瘤細胞,以TRAP-ELISA方法觀(guān)察細胞端粒酶活性,W estern-b lot檢測端粒酶蛋白的表達,細胞記數法檢測對細胞生長(cháng)的影響。Methods:A plasmid including U6 promoter and siRNA of hTERT was designed and constructed. The plasmid was transfected into ALVA-41 cell line. The telomerase activity was tested by telomerase repeat amplification protocol ELISA(TRAP-ELISA).
- 重新啟動(dòng)reboot
- 啟動(dòng)子promotor
- 人黑素瘤細胞A375 MIA/CD-RAP基因啟動(dòng)子的克隆及其鑒定Cloning of human MIA/CD-RAP promoter and its identification
- 人端粒酶逆轉錄酶基因啟動(dòng)子區的克隆及其真核表達載體的構建Cloning of human telomerase reverse transcriptase gene promoter and construction of its eukaryotic expression vector
- 可及啟動(dòng)子accessible promoter
- T7啟動(dòng)子T7 RNA polymerase promoter
- TK啟動(dòng)子TK promoter
- 基因外啟動(dòng)子extragenic promoter
- 小啟動(dòng)子minimal promoter
- A3啟動(dòng)子actin 3 promoter
- C區啟動(dòng)子core promoter
- KDR啟動(dòng)子KDR promoter
- 35S啟動(dòng)子35S promoter
- E8啟動(dòng)子E8 promoter