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- 花椰菜花葉病毒35S啟動(dòng)子cauliflower mosaic virus 35 S promoter
- 轉基因食品的檢測一般基于聚合酶鏈式反應(CR)法,對35S啟動(dòng)子和NOS終止子進(jìn)行篩選。The method was based on using the polymerase chain reaction( PCR) to determine the35 S promoter and the NOS terminator for detection of GMOs.
- 結果顯示,青枯病菌誘導的PPP1、PPP2和PPP3活性分別為35S啟動(dòng)子活性的53、39和25倍。HarpinXoo, DEG, DIR, or DPR activated PPP1 and PPP2 but not PPP3, consistent with the presence of Eli boxes in promoters.
- 以pC1303質(zhì)粒為基礎,構建了均由35S啟動(dòng)子驅動(dòng)的CMO基因和BADH基因的植物雙基因表達載體pC35SC35SB1303。A doublegene plant expression vector,pC35SC35SB1303 were reconstructed based on PC1303 plasmid by CMO and BADH which were both driven by 35S promoter.
- 我們利用此種芯片實(shí)現了對質(zhì)粒pCAMBIA1301中所含的四個(gè)基因GUS、35S啟動(dòng)子、hpt、aadA的特異性同步檢測。GUS,35S,hpt and aadA gene of plasmid pCAMBIAlSOl are simultaneously specifically detected by using arryed primer extension chip.
- 結論 改良CTAB法制備的DNA可用作PCR模板 ,建立的PCR檢測camv 35S啟動(dòng)子和nos終止子方法可用于篩選食品中有無(wú)轉基因成分S promoter,and 1%25 RRS could be indentified by PCR detecting nos te rminator. Conclusion DNA extracted from genetically modified food b y using modified CTAB methods can be used as template for PCR,the PCR method of d etecting camv 35? S promoter and nos terminator can be used for screenin g genetically modified foods.
- 啟動(dòng)子promotor
- 為了鑒定DFL基因的功能作用,構建了由35S啟動(dòng)DFL基因的正反義表達載體(載體中同時(shí)包含有由另外兩個(gè)35S啟動(dòng)的潮霉素篩選基因和GUS組織化學(xué)染色基因)轉化煙草。A full-length cDNA of DFL and its antisense nucleotide sequence have been transformed into Nicotiana tabacum under the control of a cauliflower mosaic virus 35S promoter.
- 可及啟動(dòng)子accessible promoter
- T7啟動(dòng)子T7 RNA polymerase promoter
- TK啟動(dòng)子TK promoter
- 基因外啟動(dòng)子extragenic promoter
- 小啟動(dòng)子minimal promoter
- A3啟動(dòng)子actin 3 promoter
- C區啟動(dòng)子core promoter
- KDR啟動(dòng)子KDR promoter
- 35S啟動(dòng)子35S promoter
- E8啟動(dòng)子E8 promoter
- U6啟動(dòng)子U6 promoter
- AFP啟動(dòng)子AFP promoter
