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- Methods The tumor antigen peptides from 4T1 breast cancer cells were acquired by freeze thawing method and HSP70-4T1 peptides complex was reconstructed in vitro. 方法凍融法獲取BALB/C小鼠4T1乳腺癌細胞抗原肽,體外重組HSP70-4T1抗原肽復合物。
- Recent researches have demonstrated that the ATPase domain of HSP70 and the tumor antigenic peptide that binds to Hsp70 were the crucial parts eliciting tumor-specific immunity. 最近的研究表明HSP70攜帶的小肽及其ATPase結構域是HSP70抽提物激活腫瘤特異性免疫反應的關(guān)鍵。
- Methods Tumor antigen peptides from elemene-combo Hca-F cell (HTA) combined with HSP70BCG into HTA-HSP70BCG in vitro. DCs were induced in medium with GM-CSF and IL-4 and pulsed with HTA-HSP70BCG, HTA and HSP70BCG, respectively. 方法來(lái)源于小鼠的肝癌Hca-F欖香烯復合疫苗的抗原(HTA)與卡介苗來(lái)源的HSP70(HSP70BCG)在體外構建成HTA-HSP70BCG復合物,用GM-CSF和IL-4誘導樹(shù)突狀細胞(DCs),分別用HTA-HSP70BCG、HTA和HSP70BCG對其沖激。
- Xu: It will become a tumor antigen. 徐：它會(huì )變成一種腫瘤抗原，
- In vivo and in vitro activation of CTL by murine dendritic cells pulsed with acid eluted tumor antigen peptides 弱酸洗脫提取的腫瘤抗原肽致敏的樹(shù)突狀細胞對CTL的體內外激活效應
- Cytotoxicity Induced by Dendritic Cells Pulsed with Acid Eluted Tumor Antigen Peptides against Bladder Cancer Cell Line 負載酸洗抗原肽的樹(shù)突狀細胞誘導抗膀胱癌的作用
- Methods:Human TSN,inactivated TSN and tumor antigen were prepared. 方法 :制備人肺腺癌A5 4 9細胞的TSN、滅活TSN和凍融抗原 ;
- Tumor antigenic peptide 腫瘤抗原肽
- Tumor antigen specific CTLs may prevent metastasis in the LCI-D20 model and prolong the survival time. 腫瘤抗原特異性CTL可以預防LCI-D20模型肝癌發(fā)生轉移,延長(cháng)動(dòng)物存活時(shí)間。
- Objective To study the immune response of dendritic cells (DC) modified with tumor antigen and SEB to transplanted tumor in nude mice. 摘要目的研究結腸癌患者外周血樹(shù)突狀細胞(DC)體外誘導后在裸鼠體內的抗癌效應。
- Somatostatin receptor-like immunoreactivity was in most cases located at the plasma membrane as well as in the cytoplasm of the tumor cells and was completely blocked with antigenic peptide. 良性卵巢上皮腫瘤組織中大部分細胞的胞漿和胞膜都呈強染色，呈棕褐色。
- Objective To investigate antitumor effects induced by dendritic cells (DCs) pulsed with complex of tumor antigen from elemene-combo tumor cell vaccine-heat shock protein 70 of BCG (HTA-HSP70BCG). 目的觀(guān)察體外構建的欖香烯復合瘤苗抗原-卡介苗熱休克蛋白70復合物(HTA-HSP70BCG)誘導的樹(shù)突狀細胞疫苗的抗腫瘤效應。
- Aβ1-15 multiple antigen peptide vaccine Aβ1-15多重抗原肽疫苗
- It will become a tumor antigen. 它會(huì )變成一種腫瘤抗原。
- human soluble antigen peptide 35(HS-Ag P35) 人視網(wǎng)膜可溶性抗原第35段抗原決定簇
- METHODS: BMDCs were stimulated by CpG ODN in combination with tumor antigen (TAg). The expression of CD80 on BMDC surface was analyzed by FCAS. Level of IL-12 (p70) in supernatants of BMDC culture was detected by ELISA. 方法 :CpGODN聯(lián)合腫瘤抗原 (TAg)體外刺激BMDC ,流式細胞術(shù)檢測受刺激BMDC表達CD80的陽(yáng)性率 ,ELISA檢測受刺激BMDC分泌IL - 12 (p70 )的水平 ,MTT法檢測活化BMDC刺激T細胞的增殖能力 ,聯(lián)合CpGODN和TAg體內預激小鼠獲得CTL ,檢測CTL對肝癌細胞株Hca-F的特異性殺傷作用。
- The tumor specific CTL was generated form activated autologous T cell by DC vaccine loaded with tumor antigen, and the killing activity of CTL to CT26 cells was tested by the lactate dehydrogenase (LDH) release assay in vitro. 負載腫瘤抗原的DC疫苗激活同源T淋巴細胞產(chǎn)生細胞毒性T淋巴細胞(CTL),采用乳酸脫氫酶(LDH)4h釋放法檢測CTL在體外對CT26細胞的殺傷活性;
- The binding of antigenic peptide sequences to major histocompatibility complex（MHC） molecules is a prerequisite for stimulation of cytotoxic T cell responses. 抗原多肽序列與主要組織相容性復合體(MHC)分子的結合是引起T細胞免疫應答的先決條件。
- It is critical in antitumor immune responses that the APCs (antigen presenting cells) present tumor antigens(Tags) to T cells to activate immune system to eradicate tumor cells. 抗腫瘤免疫應答中關(guān)鍵的一步是抗原遞呈細胞(APC)將腫瘤抗原遞呈給T細胞，激活機體的免疫系統來(lái)清除腫瘤細胞。 樹(shù)突狀細胞(DC)是目前認為功能最強的抗原遞呈細胞，也是APC的一個(gè)獨特的亞群。
- The adoptive transfer technique uses a small fragment of each patient's melanoma to grow T-cells in vitro targeting specific tumor antigens. 采用轉移技術(shù)利用每個(gè)患者黑色素瘤中的極少部分，體外培養特異腫瘤抗原T細胞。
