Methods Tumor antigen peptides from elemene-combo Hca-F cell (HTA) combined with HSP70BCG into HTA-HSP70BCG in vitro. DCs were induced in medium with GM-CSF and IL-4 and pulsed with HTA-HSP70BCG, HTA and HSP70BCG, respectively.
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- 方法來(lái)源于小鼠的肝癌Hca-F欖香烯復合疫苗的抗原(HTA)與卡介苗來(lái)源的HSP70(HSP70BCG)在體外構建成HTA-HSP70BCG復合物,用GM-CSF和IL-4誘導樹(shù)突狀細胞(DCs),分別用HTA-HSP70BCG、HTA和HSP70BCG對其沖激。
