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- Effect of labeling was detected by dot blot hybridization. 點(diǎn)雜交方法檢測探針標記效果。
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我們用常見(jiàn)的克隆載體pBR322作探針,用核酸點(diǎn)雜交和Southern吸印方法,檢測了來(lái)自173人的213例樣品。
- Methods: Using Western blotting hybridization assay, we detected VMLC1, VMLC2 in 13 patients with RHD and 7 cases of accidental deathes. 方法：采用West－ernbloting技術(shù)對13例RHD心衰患者及7例意外死亡者VMLC－1，VMLC－2含量進(jìn)行定量分析。
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的：探討斑點(diǎn)雜交法檢測結核分枝桿菌的臨床應用價(jià)值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑點(diǎn)雜交證實(shí)我們制備的探針是敏感而可靠的。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑點(diǎn)雜交技術(shù)鑒定分枝桿菌菌種的靈敏度為92.;49%25;特異度為100%25。
- To develop a universal PCR-reverse blot hybridization assay, which aime to detect and identify pathogenic yeast rapidly and exactly. 摘要建立PCR結合寡核苷酸探針?lè )聪虬唿c(diǎn)雜交技術(shù)，快速檢測及鑒定致病性酵母菌。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法：采用聚合酶鏈反應結合等位基因特異的寡核苷酸探針雜交（PCR/ASO）技術(shù)。
- Methods:Using digoxigenin-labeled probe,75 sera of known or unknown etiology, and 17 blood donors were detected by dot blot hybridization. 方法：用地高辛素標記基因探針，斑點(diǎn)雜交法檢測未知和已知病原的肝炎患者75例，正常對照17例。
- Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 為了探究?jì)绕に?1（ET?1）對腎功能的影響和作用方式，采用斑點(diǎn)雜交和原位雜交方法對大鼠缺血60分鐘再灌注腎組織ET?1及其受體亞型（ETA、ETB）的基因表達進(jìn)行了研究。
- Methods: The gene rearrangement in blood and bone marrow of 12 children with AML was detected by Southern blot hybridization using LE11. 8, MYO and M13 probes. 方法：用LE11.;8、MYO和M13探針，經(jīng)Southern雜交法檢測12例兒童急性粒細胞白血病患者的外周血或骨髓細胞的基因重排。
- Methods b-FGF expressions were examined by RNA dot blot hybridization and immunohistochemical staining in specimens of 40 normal prostate tissues(NP)and 38 BPH tissues. 方法采用斑點(diǎn)雜交技術(shù)和免疫組化染色測定40例正常前列腺(NP)、38例前列腺增生癥(BPH)組織中b-FGF的表達情況。
- With Southern Blotting hybridization of R plasmid DNA with gentamycin resistant gene probe from America, Australia and our lab,a different gentamycin resistant gene was found and thd new gene was cloned into pBluescript SK( I ) vector. 應用源自美國、澳洲及本室的慶大霉素耐藥基因探針對分離的慶大霉素耐藥菌株進(jìn)行Southern印跡雜交,又發(fā)現了另一種與之不同源的慶大霉素耐藥基因,并對該基因進(jìn)行了分子克隆和初步分析。
- The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf ?Fijivirus?(RBSDV). 用RT PCR技術(shù)、PCR標記的探針點(diǎn)雜交和SDS PAGE檢測了生產(chǎn)上嚴重危害玉米和水稻的水稻黑條矮縮病毒(RBSDV)。
- After incubation with Cpd 861 for 48 hours, HSC was harvested to determine collagen type I,III,IV mRNA by dot blot hybridization and cell culture medium was used to detect collagen secretion by ELISA method. 傳一代細胞以10mg/ml復方861作用48小時(shí)后 ,以ELISA法、斑點(diǎn)雜交法檢測膠原蛋白分泌及相應mRNA水平的變化。
- The FMR-1 gene mutation and Xq27.3 fragile site among 233 non-specific mentally retarded children were investigated in our genetic counseling department and two special educational schools by PCR, Southern Blot hybridization and cytogenetic methods. 采用PCR、Southern Blot印跡雜交及細胞遺傳學(xué)方法;對233名原發(fā)性智力低下患兒進(jìn)行了FMR-1基因的突變分析和Xq27.;3脆性位點(diǎn)檢查。
- Dot blot hybridization with the two probes on Nitrocellulose Membranes showed the positive results for PPV DNA from different resources and PUP recombinant plasmid,but negative for the nucleic acid samples obtained from a series of control virus. 分別對不同來(lái)源的豬細小病毒DNA及PUP重組質(zhì)粒于硝酸纖維素膜上打點(diǎn)雜交，免疫呈色后均為陽(yáng)性反應，而對照的豬瘟病毒、乙型腦炎病毒、偽狂犬病毒、PK－15細胞的核酸均為陰性反應。
- Dot blot hybridization with the two probes showed the positive result for PPV DNA,but negative for the nucleic acid samples obtained from Hog cholera virus,Pseudorabies virus,Japanese B Encephalitis virus and PK 15 cells. 對豬細小病毒DNA進(jìn)行斑點(diǎn)雜交，兩種探針均為陽(yáng)性，而對照的豬瘟病毒、豬偽狂犬病毒、乙型腦炎病毒及PK－15細胞的核酸均為陰性。
- reverse cross blot hybridization 反向雜交
