After incubation with Cpd 861 for 48 hours, HSC was harvested to determine collagen type I,III,IV mRNA by dot blot hybridization and cell culture medium was used to detect collagen secretion by ELISA method.

 
  • 傳一代細胞以10mg/ml復方861作用48小時(shí)后 ,以ELISA法、斑點(diǎn)雜交法檢測膠原蛋白分泌及相應mRNA水平的變化。
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