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- Construction of the recombinent retroviral vector rRV-hIL-4 and expression of rheumatoid arthritis in vitro. 攜帶hIL-4逆轉錄病毒重組體的構建及在類(lèi)風(fēng)濕關(guān)節炎中體外表達的研究
- After A549 was infected by recombinant retroviral vector expressing antisense ANG,ANG protein level decreased greatly. 構建出含反義血管生成素的逆轉錄病毒載體 ,感染A5 4 9細胞后 ,能有效抑制A5 4 9細胞血管生成素蛋白的表達。
- Here, we review the advance in the research of retroviral vector in the hemophilia A gene therapy. 本文對逆轉錄病毒在血友病A基因治療中的研究進(jìn)展作一綜述。
- The 3FLAG-Id3 fusion gene was amplified from p3FLAG-Id3-CMV-10 vector and subcloned into retroviral vector pMX-CITE/IRES-EGFP. 以p3FLAG-Id3-CMV-10為模板,用PCR擴增3FLAG-Id3融合基因,將其亞克隆至逆轉錄病毒載體pMX-C ITE/IRES-EGFP中;
- Recombinant retroviral vector expressing antisense ANG can efficiently inhibit ANG expression in A549 cell in vitro,providing the basis for further study in vivo. 利用反義血管生成素的逆轉錄病毒載體能夠有效抑制培養細胞血管生成素的表達 ,為今后的體內應用奠定了基礎。
- Intravenous in vivo transfection of retroviral vector bearing hRI sequence suppressed the growth of B16 melanoma inoculated in C57BL mice through antiangiogenesis mechanism. 介導hRI基因的逆轉錄病毒載體可以經(jīng)靜脈在小鼠體內轉染通過(guò)抗血管生成實(shí)現對B16黑色素瘤的抑制。 hRI在腫瘤纖維包膜中的成纖維細胞的高表達提示腫瘤發(fā)生時(shí)快速生長(cháng)的成纖維細胞可以成為重要靶點(diǎn)。
- Objective To construct recombinant retroviral vector expressing antisense ANG and transfect it into the human lung cancer cell line A549 to inhibit the expression of ANG. 目的 構建含反義人血管生成素的真核表達載體 ,并轉染人肺癌細胞株A5 4 9,使之特異性地封閉A5 4 9細胞中血管生成素的表達 ,以達到抑制腫瘤生長(cháng)的目的。
- Using a defective retroviral vector, the investigators transduced ex vivo CD34+ bone marrow cells from five boys with this SCID, resulting in no adverse effects. 調查者使用一種有缺陷的逆轉錄病毒載體，把5名重癥聯(lián)合免疫缺陷患兒的CD34+骨髓細胞取出體外并進(jìn)行轉換。
- The retroviral vector (RCAS) has been widely used in avian system to study development and diseases, but is not suitable for mammals which do not produce the retrovirus receptor TVA. 近年來(lái),鳥(niǎo)類(lèi)逆轉錄病毒載體(RCAS)及其受體(TVA)系統在哺乳動(dòng)物轉基因模型中得到廣泛應用。
- For mass production of hEGF, Bombyx mori baculovirus expression vector system was adopted in this experiment. 為了高效表達人表皮生長(cháng)因子，我們采用了家蠶桿狀病毒表達系統(Bombyx mori baculovirus expression vector system, BMBEVS)。
- Exogenous hIL 10 gene can be transferred into Rat CMC and be expressed stably. The retroviral vector PLX(hIL 10)SN shall lay foundation for researching hIL 10 mechanism and its application for curing heart diseases. 外源性hIL?10基因可以轉移到大鼠心肌細胞并穩定表達，為今后研究hIL?10的作用機制及在治療疾病中的應用打下了基礎。
- AIM: It is to investigate that apoptosis of MDA-MB-435 cell, a human breast cancer cell line, was induced by recombinant retroviral vector mediated CED-4 gene of cadherin 5 N terminal and its apoptosis mechanism. 目的:研究粘附素5 N 端CED-4(cadherin extracellular domain-4)基因經(jīng)重組逆轉錄病毒介導轉染人乳腺癌細胞MDA-MB-435 及其誘導MDA-MB-435 細胞凋亡的分子調控機制。
- Methods Green fluo rescence protein(GFP)gene was used as a reporter gene,and was transferred int o lens epithelial cells mediated by retroviral vector,GFP expression in cells w as analyzed. 方法用綠色熒光蛋白基因作為報告基因,以逆轉錄病毒載體攜之轉染晶狀體上皮細胞,觀(guān)察GFP在晶狀體上皮細胞的表達情況。
- Teaching Study for Some Concepts of Vector Systems in Linear Algebra. 線(xiàn)性代數中涉及向量組的幾個(gè)概念的教學(xué)研究。
- Objective To evaluate the bystander effect in the retrovirus vector the herpes simplex virus thymidine kinase gene Ganciclovir system (RV HSV tk GCV). 目的 研究反轉錄病毒載體 (RV) -單純皰疹病毒胸苷激酶基因 (HSV tK) -環(huán)丙氧苷 (GCV)系統中的旁觀(guān)者效應。
- self-inactivating retroviral vector 自(動(dòng))失活逆轉錄病毒載體
- Keywords bFGF;retroviral vector;gene transfer; 逆轉錄病毒載體;基因轉移;
- Keywords NT3;Retroviral Vector;DNA Recombinant; 關(guān)鍵詞NT3;逆轉錄病毒載體;基因重組;
- Methods The methods of historical analysis and textual research are used to study the evolution of the idea of a vectorial system. 方法運用文獻考證和歷史分析的方法對向量概念的演變進(jìn)行研究。
- Alfa's Q2 front-wheel torque vectoring system will also be part of the MiTo's GTA package, although a torsion-beam suspension will still be employed out back. 阿爾法的第二季度前輪扭矩矢量控制系統也將部分戶(hù)的多倫多封裝，雖然扭轉梁懸架仍然采用了回來(lái)。
