The 3FLAG-Id3 fusion gene was amplified from p3FLAG-Id3-CMV-10 vector and subcloned into retroviral vector pMX-CITE/IRES-EGFP.

 
  • 以p3FLAG-Id3-CMV-10為模板,用PCR擴增3FLAG-Id3融合基因,將其亞克隆至逆轉錄病毒載體pMX-C ITE/IRES-EGFP中;
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