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- The Restriction Enzyme Database from NEB. 限制苺資料庫取自新英格蘭生物實(shí)驗室公司。
- It enables a specific gene to be located on a particular restriction enzyme fragment. 它就能使專(zhuān)一的基因被定位于特定的限制性?xún)惹忻盖谐傻钠紊稀?/li>
- Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation. 根據揭示多態(tài)性的限制性?xún)惹忻傅臄盗靠蓪a(chǎn)生的突變大多歸為點(diǎn)突變。
- Certain DNA sequences oriented within this gap are substrates for Alu I, a blunt end restriction enzyme. 如果用一段酶切斷作為橋梁的DNA，那么整個(gè)電路的電流也被中斷了。
- Amplified of the green fluent protein (GFP) gene from plasmid PCR3.1, and add suitable restriction enzyme cutting site. 從含有g(shù)fp基因的質(zhì)粒pCR3.;1上擴增出全長(cháng)基因，并且加上合適的酶切位點(diǎn)。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 結果：酶切鑒定及測序結果提示表達產(chǎn)物正確；
- The expressive vector pcDNA3.1(-)-ASL has been constructed and had been confirmed by restriction enzyme cut and sequencing. 構建的真核表達載體pcDNA3 .;1(-) ASL經(jīng)過(guò)酶切鑒定和測序證實(shí)正確。
- Genomic DNA was extracted.Polymerase chain reaction, direct sequencing and restriction enzyme reaction were performed to identify the mutations. 利用聚合酶鏈反應、DNA直接測序進(jìn)行突變檢測,進(jìn)一步采用限制性?xún)惹忻该盖序炞C突變。
- Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析篩選出保護性抗原基因片段的重組子,從而制備成芯片探針。
- The vector was identified via PCR and restriction enzyme analysis and was introduced into Agrobacterium tumerifacience LBA4404. 經(jīng)限制性?xún)惹忻阜治龊蚉CR鑒定后利用凍融法將重組質(zhì)粒pBI121-A導入根癌農桿菌LBA4404。以錦橙 (Citrus.
- The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP). 用聚合酶鏈反應-限制性片段長(cháng)度多態(tài)性方法,分析限制性酶切位點(diǎn)-48A/的多態(tài)分布。
- Methods: The serum samples were collected from 56 patients with morbilliform maculopapular eruption.The target DNA of CBV was detected by RT-PCR and restriction enzyme analysis. 方法:取56例春季流行性麻疹樣發(fā)疹患者外周血標本,擴增目的DNA并進(jìn)行特定限制性?xún)惹忻阜治觥?/li>
- Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 結果 1、對重組載體的酶切鑒定和測序結果證實(shí)，在pS2-DTA中EWS-FLI-1特異性結合序列(ACCGGAAGT)位于白喉毒素A鏈基因上游。
- The ORF of two circadian gene BMAL1 and CRY1 were cloned from normal human whole brain marathon cDNA and verified with restriction enzyme digestion and DNA sequencing. 從正常人全腦marathon cDNA中克隆到人生物節律基因BMAL1和CRY1的開(kāi)放讀碼框架(ORF)，經(jīng)酶切和測序鑒定與GeneBank數據庫中收錄的序列基本一致。
- Restriction endonuclease (restriction enzyme) A type of enzyme, found mainly in bacteria, that can cleave and fragment DNA internally(see endonuclease). 限制性?xún)惹忻福ㄏ拗泼福褐饕诩毦写嬖诘囊环N酶類(lèi)，它可以從DNA的內部將其切開(kāi)和分裂。
- Methods Polymerase chain reaction with restrict enzyme digestion was used to detect the target gene polymorphism in 78 normotension controls and 72 EH patients. 目前認為，高血壓是多種遺傳因素和環(huán)境因素共同作用而引起的復雜性狀疾病，在人類(lèi)遺傳易感性的基礎上，在環(huán)境危險因素的作用下發(fā)病。
- New phages can develop by acquiring restriction enzymes from plasmids. 通過(guò)從質(zhì)粒獲得的限制性酶,新的噬菌體能夠生長(cháng)。
- Cut outside restricted enzymatic what be? 限制性外切酶是什么呀？
- Restriction enzymes and reverse transcriptase are keys to gene engineering. 限制性酶和逆轉錄酶成為基因工程的重要手段。
- Obtaining of SPCEMA(signal peptide modified CEMA)SPCEMA(187bp) was amplified with two long complementary primers (P2 and P3) and two primers (Pl and P4) containing restriction enzyme recognition site. 以?xún)蓷l部分重疊長(cháng)鏈引物P_2和P_3延伸產(chǎn)物為模板，P_1和P_4為正、反引物進(jìn)行PCR擴增，獲得了改造的抗菌肽基因SPCEMA(187bp)。
