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- Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast.Since 1980’s, it has been deve loped a lot. 酵母基因中斷技術(shù)是研究酵母基因功能的重要手段，自80年代初誕生以來(lái)經(jīng)歷了不斷的改進(jìn)和發(fā)展.
- Abstract： Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast.Since 1980’s, it has been deve loped a lot. 摘 要： 酵母基因中斷技術(shù)是研究酵母基因功能的重要手段，自80年代初誕生以來(lái)經(jīng)歷了不斷的改進(jìn)和發(fā)展.
- The gene function was studied by using the method of gene disruption and the result showed that the sawl played an important role in developmental changes from aerial hypha to spore formation of Streptomyces ansochromogenes. 用基因破壞的策略進(jìn)一步研究了該基因的生物學(xué)功能;結果表明saw1在圈卷產(chǎn)色鏈霉菌氣生菌絲到孢子形成的發(fā)育轉變中有重要作用;是分化中控制孢子發(fā)育起始的一個(gè)重要基因.
- The vector of narG gene targeting pEGude was introduced into denitrifier DNF409 by electrotransformation. An Erm~r Cm~s isolate DNF409EF was obtained and narG gene disruption was confirmed by PCR. 將基因中斷載體pEGude電激轉化至反硝化菌DNF409中，通過(guò)抗性篩選得到表型為Erm~r Cm~s的重組子DNF409EF，并通過(guò)PCR分析證實(shí)narG基因已被正確中斷。
- PCR-mediated gene disruption technique makes the manipulation easi er and it can be used to precisely delete genes in yeast. PCR介導的酵母基因中斷技術(shù)，大大簡(jiǎn)化了操作，實(shí)現了酵母基因的精確缺失；
- Research Advances of Yeast Gene Disruption 酵母基因中斷技術(shù)研究進(jìn)展
- Keywords Geldanamycin;Biosynthetic gene;Genomic library;Gene disruption; 生物合成基因;基因文庫;基因阻斷;
- Keywords Streptomyces tenebrarius;carbamoyltobramycin;the biosynthetic gene （cluster）;gene disruption; 黑暗鏈霉菌;基因阻斷;接合轉移;基因突變;
- Keywords quinic acid,biosynthesis,shikimate pathway,heterogenous enzyme,co-expression,gene disruption,gene integration; 奎尼酸;生物合成;莽草酸途徑;異源酶;串聯(lián)表達;基因破壞;基因整合;
- Keywords Daunorubicin;4'-epi-Daunorubicin;C4 Ketoreductase;Gene Disruption;Function Compensation; 柔紅霉素;表柔紅霉素;C4酮基還原酶;基因阻斷;功能補償;
- Keywords dnmV Daunorubicin Homologous recombination Gene disruption Function compensation; 柔紅霉素;同源重組;基因阻斷;功能補償;
- Keywords S.tenebrarius;Apramycin;Biosynthesis genes;Gene disruption; 黑暗鏈霉菌;安普霉素;生物合成基因;基因阻斷;
- Keywords Amycolatopsis orientalis HCCB10007;Vancomycin;Protoplast;Transformation;Gene disruption;Streptomyces griseus IFO 13350;A-factor;Grixazone;Biosynthesis.; 東方擬無(wú)枝酸菌HCCB10007;萬(wàn)古霉素;原生質(zhì)體;轉化;基因破壞;灰色鏈霉菌;A因子;黃色色素:生物合成;
- The position that a given gene occupies on a chromosome. 基因座在一條染色體上某種給定基因所占的位置
- Half of the people who inherit the gene express it. 半數有這種遺傳基因的人，皆顯示出其影響
- Now the variant of each gene is called an allele. 每一種基因的變化就叫做等位基因。
- We know a lot about the structure of gene now. 如今我們對基因的結構有了較多的了解。
- A single gene may have many effects. 單一基因可能具有很多種效應。
- There can be more than two alleles for a gene. 一個(gè)基因可能有兩個(gè)以上的等位基因。
- A mutable organism; a mutable gene. 一個(gè)易變的生物；一個(gè)易變的基因
