The vector of narG gene targeting pEGude was introduced into denitrifier DNF409 by electrotransformation. An Erm~r Cm~s isolate DNF409EF was obtained and narG gene disruption was confirmed by PCR.
英
美
- 將基因中斷載體pEGude電激轉化至反硝化菌DNF409中，通過(guò)抗性篩選得到表型為Erm~r Cm~s的重組子DNF409EF，并通過(guò)PCR分析證實(shí)narG基因已被正確中斷。
