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- Plasma membrane proteins of sperm cells from Zea mays were passed through affinity column, separated by SDS|PAGE and stained using Con A|HRP. 用親和層析,SDS?PAGE 及印跡技術(shù),從玉米精細胞中分離純化質(zhì)膜蛋白。
- MethodsMonoclonal antibody is an IgG1 antibody, sepharose 4B and SPA affinity column were applied for purifi cation of AchE monoclonal antibody (3F3), using papain cut 3F3,to get Fab fragme nt and immune mice with Fab. 方法 AchE單克隆抗體是一種IgG1抗體 ,先用木瓜蛋白酶酶切 ,然后采用AchE Sepharose 4B和SPA親和層析柱進(jìn)行提取純化 ,獲得純化的單克隆抗體Fab段。
- Results 98% purity was reached through 14F7 monoclonal antibody column, and 95% purity was gained after goat anti human Fab fragment column. But yield ratio of the two affinity columns was low, being 35% and 55%, respectively. 結果3種純化方法中,14F7單克隆抗體柱純化的Fab抗體的純度達98%25左右,Fab抗體柱純化的Fab抗體的純度為95%25,但這兩種親和柱的目的蛋白收率都不高,分別為35%25、55%25。
- The potential of sample clean-up with sol-gel affinity columns is demonstrated giving applications in environmental, food and clinical analysis. 以這些親生物性溶膠管柱來(lái)作樣品清理的應用潛力,已由環(huán)境、食物及醫療上獲得證明。
- She angled her column of chitchat toward teenagers. 她的漫談欄側重于十幾歲的青少年。
- She has a strong affinity for Beethoven. 她酷愛(ài)貝多芬的樂(lè )曲。
- Peter has a special affinity for booze. 彼得嗜酒。
- He felt a strong affinity to the Russian girl. 他深受那個(gè)俄國姑娘吸引。
- He used to write a column for this newspaper. 他過(guò)去曾為這家報紙寫(xiě)專(zhuān)欄文章。
- She feels a strong affinity to him. 她感到他對她有很強的吸引力。
- His column is syndicated throughout the world. 他的專(zhuān)欄文章通過(guò)報業(yè)辛迪加在世界許多報刊上發(fā)表。
- Italian has a close affinity to French. 義大利語(yǔ)和法語(yǔ)很相似。
- The column is surmounted by a statue. 柱頂上樹(shù)立了一尊雕像。
- Nelson's Column is a famous monument in London. 納爾遜紀念碑是倫敦著(zhù)名的紀念碑。
- He always read the sports column in the Times. 他總是閱讀《泰晤士報》的體育欄。
- Keywords high performance liquid chromatography;immune affinity column;albuterol;plasma; 高效液相色譜法;免疫親和柱;沙丁胺醇;血漿;
- The top part of a pillar or column. 柱頭一根柱子或圓柱的頂端部分
- There is an affinity between Chinese and Japanese character. 漢字與日文之間有密切關(guān)系。
- A column in the periodic table of the elements. 屬,族元素周期表上的一欄
- The column, four abreast, stretched out over one kilometre. 隊伍4個(gè)人一行,長(cháng)達1公里以上。