MethodsMonoclonal antibody is an IgG1 antibody, sepharose 4B and SPA affinity column were applied for purifi cation of AchE monoclonal antibody (3F3), using papain cut 3F3,to get Fab fragme nt and immune mice with Fab.
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美
- 方法 AchE單克隆抗體是一種IgG1抗體 ,先用木瓜蛋白酶酶切 ,然后采用AchE Sepharose 4B和SPA親和層析柱進(jìn)行提取純化 ,獲得純化的單克隆抗體Fab段。
