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- Electrophoresis mobility shift assay(EMSA)showed that both NPs of Z10 and Z37 hantaviruses bound specifically to the double-stranded DNA probe possessing the 18-nucleotide inverted repeat sequences in vitro. 然后將該探針與純化的Z10、Z37株的核蛋白NP進(jìn)行非變性凝膠電泳遷移改變實(shí)驗(EMSA)后發(fā)現,重組的Z10、Z37株的核蛋白NP,在體外均可特異地結合其基因組末端反向重復序列形成的雙鏈探針。
- Electrophoresis mobility shift assay 電泳遷移率變動(dòng)分析
- The biological activity of renatured AIF protein was detected by electrophoretic mobility shift assay (EMSA) and Hoechst staining. 采用凝膠滯后實(shí)驗(EMSA)與Hoechst 33258染色檢測AIF蛋白的生物學(xué)活性。
- Electrophoretic mobility shift assay (EMSA) and competition tests also demonstrated its binding activity to the target element. 體外凝膠電泳阻抑分析及競爭實(shí)驗亦證明該蛋白質(zhì)與沉默子DNA存在相互作用。
- The changes of blood pressure, whole blood viscosity and red blood cell (RBC) electrophoretic mobility (EPM) of all the three groups were determined. 方法:將大鼠隨機分成對照組、燙傷組和駐極體治療組,分別測量對照組、燙傷組和駐極體治療組大鼠血壓、全血粘度和紅細胞電泳率的變化。
- Reading occupies most of my free time. 閱讀占去了我大部分的閑暇時(shí)間。
- The oxidative modification of LDL was identified by agarose gel relative electrophoretic mobility(REM)and fluorescence of thiobarbituric acid reaction substances(TBARS)contents. 測定LDL的電泳遷移率及硫代巴比妥酸反應物質(zhì)含量。
- The electrophoretic mobility shift assay (EMSA, gel shift, gel retardation) is a relatively simple and sensitive method to investigate protein:DNA interactions. 電泳凝膠遷移分析是一個(gè)相對簡(jiǎn)單和靈敏的研究蛋白:DNA相互作用的方法。
- This fluorescence-based Electrophoretic Mobility Shift Assay (EMSA) Kit provides a fast, easy and quantitative method to detect both nucleic acid and protein in the same gel. EMSA試劑盒提供快速、簡(jiǎn)單和定量的方法在同一塊凝膠中檢測核酸和蛋白,在標準的電泳凝膠遷移中獲得雙倍的信息。
- Under the conditions of low surface potential and weak applied electric field, the electrophoretic mobility of a particle is evaluated by a superposition method. 在低表面電位、弱外加電場(chǎng)與低雷諾數的假設下,吾人利用加疊原理求解相關(guān)電場(chǎng)與流場(chǎng)之線(xiàn)性化方程組,并獲得膠體粒子的電泳速度(或電泳遷移率)。
- Particle electrophoretic mobility (PEM) changes are shown to correlate well with the amount of ligand fixed on the particles, as probed by its biological activity. 通過(guò)對配體生物活性的探測,發(fā)現粒子電泳遷移率(PEM)的變化與固定在顆粒上的配體數量密切相關(guān)。
- The crew remained free from scurvy. 那些船員仍然沒(méi)有患壞血病之虞。
- He's a fervent believer in free speech. 他是言論自由的強烈信仰者。
- She picked up a new magazine with her free hand. 她用空著(zhù)的一只手拿起一本新雜志。
- With the enzymes, however, one may add to the criteria of homogeneity in solubility behaviour, sedimentation, and electrophoretic mobility, the criterion of maximal enzyme activity and freedom from other enzymatic activities. 然而,對于酶而言,除了考慮溶解過(guò)程的均勻性,沉降作用及電泳淌度這些標準外, 還應該考慮最大酶活力和不參與其它酶的活動(dòng)這一條標準。
- Jon sent away for a free almanac. 喬寫(xiě)信去要一份免費贈閱的年歷。
- Now that I am free, I can enjoy music for a while. 既然有空,我可以享受一下音樂(lè )。
- Electrophoretic mobility shift assay further indicated the HAG IL 6RE homologous to IL 6 responsive element at -568 site of the angiotensinogen promoter binds C/EBP(CAAT/Enhancer Binding Protein). 結果IL-6使血管緊張素原mRNA明顯提高,位于血管緊張素原基因啟動(dòng)子中的HAGIL-6RE結合于轉錄因子CAAT/增強子結合蛋白(C/EBP);
- We measured and analyzed the electrophoretic mobility,osmotic fragility,membrane fluidity,fourier-transform infrared(FT-IR) spectrometer and viscoelastic properties of pDCs,imDCs and mDCs by microrheogical methods. 應用微觀(guān)流變學(xué)研究方法對以上細胞的電泳率、滲透脆性、膜流動(dòng)性和傅立葉紅外光譜(FT-IR)進(jìn)行測量和分析。
- A free flow of water came from the pipe. 水從管道里通暢地流了出來(lái)。