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- Abstract:Selective marker gene is usually used to select transformed cells or tissue during gene transfer. 在基因轉移過(guò)程中，人們常常使用標記基因來(lái)篩選轉化細胞或組織。
- There are several unique restriction sites on each selective marker,AhdI, PstI, PvuI sites on the marker of Apr and Psp, EcoRI, NcoI on the marker of Cmr. 該質(zhì)粒具有Apr和Cmr兩個(gè)抗性標記,在其氨芐青霉素抗性基因上有AhdI、PstI、PvuI插入位點(diǎn),在氯霉素抗性基因上有Psp、EcoRI、NcoI插入位點(diǎn)。
- The presence in transgenic plants of antibiotic and herbicide resistant selective marker genes might be an unpredictable hazard to the ecosystem as well as to human health. 摘要轉基因植物中的除草劑和抗生素抗性標記基因潛在的生態(tài)環(huán)境和食用安全性令人擔憂(yōu)。
- The results from RT-PCR indicated the knockdown of mRNA level of hCAP-E and hCAP-C in the co-transfeted Hela cells. We also conducted the co-transfection with the RNAi plasmids RHE and RHC and the selective marker GFP expressing plasmids simultaneously. 進(jìn)行RT一PCR檢測表明人集縮素SMC亞基hCAP一E和hCAP一C的C一末端真核表達質(zhì)粒在Hela細胞內得到過(guò)表達。
- Abstract: To improve the efficiency of targeted gene replacement (TGR), a dual screen (DS) system with gusA gene as negative selective marker (GUS-DS) was developed in Magnaporthe oryzae. 摘 要: 目標基因替換是基因功能研究的重要方法, 在生物工程領(lǐng)域廣泛應用。
- In this assay,we studied the possibility of raccoon pox virus (RCN) TK gene as a viral selection marker on Rat-2 cells. 本文在Rat-2(TK~-)細胞上,對浣熊痘病毒TK基因作為病毒篩選標記的作用進(jìn)行了研究。
- In plant genetic engineering, selectable marker is needed to distinguish transformant. 在植物基因工程中，需要用到選擇標記基因以便篩選轉化子。
- In order to increase the safety of transgenic plants, Biologists began to search for biosafe selectable marker. 為了提高轉基因植物的安全性，生物學(xué)家們開(kāi)始尋找生物安全的選擇標記。
- Objective To establish a protein expression system using pyrG gene as a selection marker and Aspergillus niger (A.niger) as a host. 目的 建立以pyrG基因為選擇標志 ,以黑曲霉菌為宿主菌的蛋白質(zhì)表達系統。
- A new strategy that offers a approach for the elimination of those disadvantages caused by the selectable marker gene is developed. 為了消除這些弊端，一種全新的發(fā)展策略即獲取無(wú)選擇標記的轉基因植物應運而生。
- These selection marker systems are potential for variety of application in food fermentations or live vehicles and so on. 雙質(zhì)粒選擇標記系統構建的篩選過(guò)程新穎獨特，為整個(gè)選擇標記系統的發(fā)展開(kāi)辟了新的途徑及思路.
- As the commercialization of transgenic plants, people are more and more paying close attention to their safety, among which refers to the safety of selectable marker. 隨著(zhù)轉基因植物的商品化，人們越來(lái)越關(guān)注其安全性問(wèn)題，其中主要涉及選擇標記的安全性。
- There was an enormous cat crouching on the counter. 柜臺上蜷伏著(zhù)一只碩大的貓。
- Study of selectable markers in genetic engineering of Chlorella sp. 小球藻基因工程選擇標記研究。
- Currently used plant transformation systems require selectable marker genes encoding antibiotic or herbicide resistance, along with the gene of interest, to select transformed cells from a large population of mostly untransformed cells. 摘要在目前的植物轉化系統中，要求在關(guān)注基因或目的基因轉入細胞時(shí)，同時(shí)有標記基因存在。
- He placed the money on the counter. 他把錢(qián)放在柜臺上。
- Can the goods be refunded at your counter? 你們柜臺的商品可以退嗎？
- One is the utilization of bio-safe positive selective markers which are genes mainly related to metabolism of auxins and carbohydrates. 一是選用安全的正向選擇標記，主要是與糖代謝和激素代謝相關(guān)的基因。
- We should not act counter to our promise. 我們不應違背自己的諾言。
- He tipped the money in his pocket onto the counter. 他把口袋里的錢(qián)傾倒在柜臺上。
