The results from RT-PCR indicated the knockdown of mRNA level of hCAP-E and hCAP-C in the co-transfeted Hela cells. We also conducted the co-transfection with the RNAi plasmids RHE and RHC and the selective marker GFP expressing plasmids simultaneously.
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- 進(jìn)行RT一PCR檢測表明人集縮素SMC亞基hCAP一E和hCAP一C的C一末端真核表達質(zhì)粒在Hela細胞內得到過(guò)表達。