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- cerb-B1基因cerb-B1 gene
- 說(shuō)明本研究構建的針對cerb-B1基因的質(zhì)粒表達載體可以顯著(zhù)抑制cerb-B1基因和EGFR蛋白在人結腸癌LoVo細胞的表達,為結腸癌的基因治療提供了新的思路。The plasmid expression vector aiming directly at cerb-B1 gene might suppress cerb-B1 gene and EGFR expression in human colon cancer LoVo cells. The new therapeutic modalities could be used in the treatment for human colon cancer.
- 目的:體外構建編碼人類(lèi)基因cerb-B1短發(fā)卡狀RNA(shorthairpinRNA,shRNA)的質(zhì)粒表達載體,觀(guān)察其對結腸癌LoVo細胞cerb-B1基因和表皮生長(cháng)因子受體(EGFR)蛋白的特異性抑制作用。Objective:To establish a plasmid expressive vector coding short hairpin RNA(shRNA) of human cerb-B1 gene in vitro, and to investigate specific inhibition of cerb-B1 gene and epidermal growth factor receptor(EGFR) expression in human colon cancer LoVo cells by shRNA.
- B1基因B1 gene
- hUNC93-B1基因hUNC93-B1 gene
- Cerb-B-2Cerb - B - 2
- 細胞周期素B1基因cyclin B1
- 基因代代相傳。Genes are transmitted from one generation to another.
- 應用脂質(zhì)體Lipofectamine2000轉染人結腸癌LoVo細胞,轉染成功后以G418篩選4周,實(shí)時(shí)熒光定量RT-PCR(RealTimeRT-PCR)檢測cerb-B1基因的mRNA表達,Western-blot檢測EGFR蛋白的表達。The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method.
- Wx-B1Wx-B1
- 基因外顯率penetrance of gene
- V．B1維生素B1, 硫胺素, 硫胺, 維生素乙1, 維他命乙1, 抗神經(jīng)炎素[維生素類(lèi)藥]
- 分離的基因型a segregant genotype.
- 基因的區分繁殖differential perpetuation
- Ｃ基因C gene
- Ｄ基因D gene
- Ｊ基因J gene
- Ｖ基因V gene
- Ｉｒ基因Ir gene
- 免疫遺傳學(xué)免疫學(xué)中研究免疫反應的細胞和基因基礎的分支The branch of immunology that deals with the molecular and genetic bases of the immune response.
