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- T7RNA聚合酶表達系統T7 RNA polymerase expression system
- T7RNA聚合酶T7 RNA polymerase
- 自H5N1陽(yáng)性病料中提取總RNA,反轉錄后采用高可信度DNA聚合酶,經(jīng)PCR擴增NP基因,采用Invitrogen定向表達系統進(jìn)行克隆表達,純化獲得N末端攜帶多聚組氨酸標簽的重組NP蛋白,分子質(zhì)量約66.0ku。Total RNA was extracted from positive clinical sample of H5N1 subtype of avian influenza virus, NP gene was amplified by reverse transcriptase-polymerase chain reaction using high proofreading polymerase (PyobestTM DNA Polymerase), and expressed using Invitrogen championTM pET directional TOPO expression system. Recombinant protein containing polyhistidine (6xHis) tag in N-terminal was about 66.0 ku in size, had been obtained and purified.
- ＤＮＡ聚合酶DNA polymerase
- T4DNA聚合酶T4DNA polymerase
- 淋巴系統lymphatic system
- 磷酸酶phosphatase
- II型系統type-II system
- ０Ｌ系統zero-sided Lindenmayer system
- Ｔ系統transverse tubular system
- (使)聚合polymerize
- Ｑ酶branching enzyme
- ２Ｄ系統2D system
- 高聚合凝聚劑high polymer coagulant
- ＭＫ系統MK system
- [生化]合酶synthase
- 磷酸聚合phosphoric acid polymerization
- ＤＮＡ酶deoxyribonuclease
- ＲＣ系統Ritchey-Chretien system
- ＲＮＡ酶ribonuclease
