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- Southern blot analysis showed that Oncidium has at least 4 copies of EIN2 in its genome. 南方墨點(diǎn)分析顯示EIN2至少有四個(gè)拷貝分布于基因體組內。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑點(diǎn)雜交法和 Southen 印跡法對腦瘤中 erbB、sis、myc 和 fos 這四種癌基因的擴增和重排進(jìn)行了研究。
- PCR assay and Southern blot analysis showed that CMO gene was integrated successfully into the genome of transformed plant. PCR及Southern檢測證明CMO基因已整合進(jìn)抗性植株基因組;
- Southern blot analysis and PCR of target segment in the genome of tobacco showed that all of the plants generated were the transgenic plants. 轉基因植株總DNA PCR和Southern blot分析表明目的基因已經(jīng)整合到植物基因組中,但轉基因的拷貝數與抗病性無(wú)明顯的相關(guān)性。
- DNA dot blot analysis showed that 73. 4% of resistant plants contained GNA and hpt genes. Southern blot analysis confirmed the integration of GNA gene in the genome of transgenic rice. DNA點(diǎn)雜交檢測表明73.;4%25的抗性植株同時(shí)含有GNA基因和hpt基因;Southern雜交分析進(jìn)一步證實(shí)了GNA基因在轉基因水稻基因組中的整合
- Organogenic transgenic calli and transgenic regenerated plantlets were produced on selection medium containing 15mg/L kanamycin, and confirmed by GUS histochemical staining, polymerase chain reaction (PCR), and southern blot analysis. 器官發(fā)生的轉基因愈傷組織和轉基因的再生植株已經(jīng)獲得 ,并經(jīng)GUS組織化學(xué)染色、聚合酶鏈式反應和Southern雜交分析證實(shí)。
- Dot blot and southern blot analyses suggested that gfp gene is integrated into the genome of transgenic plants of Nicotiana tabacum, Pentunia genome. 通過(guò)對有綠色熒光的煙草、矮牽牛進(jìn)行dot blot、southern blot分析，都獲得陽(yáng)性雜交結果，證實(shí)gfp基因已整合到植物基因組中并與綠色熒光觀(guān)察結果一致。
- Southern blotting analysis of genomic DNA indicated that enolase was likely to be a low-copy gene in the oilseed rape genome. Southern雜交結果顯示烯醇酶以低拷貝形式在油菜基因組中存在.
- The expression vector was linearized and transformed protoplasts of Aspergillus niger strain An1. Hygromycin-resistant transformants were generated and the integration of xylanase gene was confirmed by genomic Southern blotting analysis. 將該表達載體線(xiàn)性化后轉化果膠酶產(chǎn)生菌黑曲霉菌株An1的原生質(zhì)體。 對抗潮霉素黑曲霉轉化子進(jìn)行基因組Southern雜交分析結果表明,木聚糖酶基因已整合到受體基因組中。
- Using Southern Blot Analysis to Determine the Integration of Transduced Genes in the Genomic DNA of Host Cells 應用Southern印跡雜交法檢測轉移基因在宿主細胞中的整合
- fps gene probe was signed with DIG High Prime, PCR- Southern blot analysis with PCR production of fps gene, proved fps gene was expressed in molecule level. 利用地高辛標記fps基因探針，對fps基因PCR產(chǎn)物進(jìn)行PCR-Southern檢測，并且檢測到了目的基因片段的印跡，證明fps基因在發(fā)狀根基因組分子水平得到了表達；
- Southern blot technique developed to identify DNA fragments. 印跡雜交被用來(lái)發(fā)現DNA片段。
- This CTB gene product was also verified by Western blot analysis. Western blotting結果確認了該條帶為CTB基因的產(chǎn)物。
- The results of western blot analysis were in accordance with those of 2-DE. 對部分差異蛋白采用Western-blot方法在蛋白水平的驗證結果與2-DE結果基本相符。
- Regenerated plants with kanamynic resistantance were obtained. PCR , PCR-Southern blot analysis , southern dot blot analysis of lettuce DNA confirmed that adw gene had been integrated into the plant genome. 細胞核載體PB-adw、PBG-adw均采用農桿菌介導法將adw導入萵苣，細胞核轉化獲得了生長(cháng)良好的抗性萵苣植株，經(jīng)PCR、PCR-Southern、southern斑點(diǎn)雜交分析證實(shí)，adw基因已整合到萵苣基因組中。
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我們用常見(jiàn)的克隆載體pBR322作探針,用核酸點(diǎn)雜交和Southern吸印方法,檢測了來(lái)自173人的213例樣品。
- Southern Blot with positive plants of PCR showed that CMO and BADH have been integrated into the rice genome. 對隨機抽取的PCR檢測雙陽(yáng)性植株進(jìn)行Southern blotting雜交檢測分析，表明CMO和BADH基因均已整合到轉基因水稻的基因組中。
- Transgenic potato plants 1M, 2M, 3M, 5M, 10M, llM provided by laboratory were analyzed by Southern blot and Northern blot. ELISA檢測轉基因馬鈴薯病毒抗性數據顯示，溫室條件下，3個(gè)轉基因馬鈴薯株系(2M、3M、11M)具有病毒抗性，3個(gè)株系(1M、5M、10M)感??；
- RT-PCR, Western blot analysis and FACS were used to detect the expression of P2X7 receptor in these clones. 熒光分光光度計檢測P2X7受體介導的胞內鈣離子濃度變化。
- Using morphology observation, the RT-PCR and Western blot analysis to investigate and analysis the sub-type of CnA. 通過(guò)病理形態(tài)學(xué)檢查、RT-PCR實(shí)驗測定及Western blot分析確認CnA亞型。
