The expression vector was linearized and transformed protoplasts of Aspergillus niger strain An1. Hygromycin-resistant transformants were generated and the integration of xylanase gene was confirmed by genomic Southern blotting analysis.
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- 將該表達載體線(xiàn)性化后轉化果膠酶產(chǎn)生菌黑曲霉菌株An1的原生質(zhì)體。 對抗潮霉素黑曲霉轉化子進(jìn)行基因組Southern雜交分析結果表明,木聚糖酶基因已整合到受體基因組中。
