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- 脈絡(luò )寧組40例(男31例,女9例;年齡62±s8a)用脈絡(luò )寧20mL+10%葡萄糖500mL,靜滴,qd,15d為一個(gè)療程,用2個(gè)療程程。Mailuoning(MLN) group including 40 patients (M 31. F 9; age 62+ Sa) was given MLN 20 mL in 10%25 glucose 500 mL, iv, qd, 15 d as a course of treatment;
- 最佳發(fā)酵條件為:培養溫度:25℃,起始pH:5.0,500ml三角瓶裝液量為200ml,搖床轉速:150r/min,發(fā)酵培養12天。The most moderate breeding condition for fermentation is: Temperature 25C,the original pH for medium is 5. 0, The volume of fermentation solution is 200ml in 500ml Erlenmeyer flash , the rocking bed velocity is 150r/min, fermentation time is 12 days.
- 后一種方法是把195g PbO加到500ml 2mol/L的高氯酸溶液中,再通入電流密度為2.5mA/cm2的電流,取出沉積物,將其搗碎并用水洗凈。In the latter method lead monoxide (195g) was added to 500 ml of 2M perchloric acid solution and a current of density 2.5mA cm-2 was passed. The deposit was removed, ground, and washed with water.
- 對表達人骨形成蛋白?2A(BMP?2A)的重組大腸桿菌YK537/pDH?B2m在500ml搖瓶中進(jìn)行了培養條件的摸索實(shí)驗,繼后用5L自控發(fā)酵罐進(jìn)行分批培養和分批補料培養,以獲取rhBMP?2A。The optimization of cultivation condition in 500ml shake flasks was carried out to produce recombinant human bone morphogenetic protein 2A(BMP 2A)in recombinant Escherichia coli YK537/pDH B2m,followed by 5L fermentor batch and condition controlled fed batch culture to obtain BMP 2A.
- 結果表明,其最佳浸提方案是:成品茶樣經(jīng)粉碎過(guò)40目(0.45mm)篩,稱(chēng)取樣品3g茶樣用400ml沸蒸餾水沖泡,置于100℃恒溫水浴鍋中浸提30min后抽濾定容至500ml,而后用茚三酮比色法測定其含量。Results showed that the best extracted design was: the baked green tea was sifted through a 0.45 mm sieve, the 3 g of sample tea were infused by 400 ml boiling deionized water, and extracted in boiling water bath for 30 min and then the extraction was sucked with suction pump and settled to the volume of 500 ml. The FAA contents were determined by the ninhydrin colorimetric method.