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- 用Cfol和BamHI將含有E2基因的3.2kb片段從HPV16基因組中切出來(lái),用S1核酸酶修平末端;coli. The 3.2kb fragment containing the E2 gene was cut out from HPVl6 genome and blunted with nuclease S1. The plasmid pBD2 DNA was linearized with Hindi and blunted with nuclease S1 too.
- 采用Trizol試劑對病毒進(jìn)行了核酸提取,電泳結果發(fā)現有4條核酸條帶,分別為3.0kb、1.3kb、0.8kb、0.75kb,對RNA核酸酶、S1核酸酶敏感,對DNA酶抵抗,為單鏈RNA病毒。The nucleic acid of virus was extracted by using the Trizol reagents and analyzed with 1%25 agarose electrophorosis. It shows the nucleic acid has four bands sized 3.0, 1.3, 0.8 and 0.75 kb respectively, and is sensitive to RNase and S1 nuclerase and resistant to DNase, indicating these bands as single?strand RNA.
- 核酸酶水解核酸的酶，如核酸內切酶和核酸外切酶Any of several enzymes, such as endonucleases and exonucleases, that hydrolize nucleic acids.
- 酶的enzymatic
- 識別序列為非回紋對稱(chēng)結構限制核酸酶的正確切割位點(diǎn)The True Recognizing Sequence of the Restriction Enzyme Whose Recognizing Sequence is Nonpalindromic
- 輔酶coenzyme
- 酶解zymohydrolysis
- 過(guò)氧化氫酶catalase
- 金黃色葡萄球菌核酸酶在大腸桿菌中的表達及其活性分析Expression and nuclease activity analysis of staphylococcus nuclease in E. Coli
- 酶活性enzyme activity
- 禽腺病毒貴州分離株HS-1的核酸酶切分析與蛋白抗原鑒定Antigenicity identification and DNA restriction map analysis of Guizhou isolate HS-1 strain
- 核酸酶P1nuclease P1
- S-核酸酶S-RNase
- Ｓ１核酸酶作圖S1 nuclease mapping
- 限制性核酸內切酶restriction endonuclease
- 核酸酶nuclease
- 脾外切核酸酶spleen phosphodiesterase
- BAL 31核酸酶BAL 31 nuclease
- S1核酸酶S1 nuclease
- Ter核酸酶Ter nuclease
