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- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 應用完整細胞原位斑點(diǎn)印跡雜交技術(shù)檢測c-myc、野生型p53(wtp53)、p16和EGFR的基因表達;
- After random labeling with DIG, RNA dot blot was proceed to test the expression of this gene under different growth condition. 用地高辛隨機引物標記試劑盒對該片段進(jìn)行標記后,對不同水分和硫營(yíng)養條件下提取的小麥根系總RNA 進(jìn)行斑點(diǎn)雜交。
- Methods b-FGF expressions were examined by RNA dot blot hybridization and immunohistochemical staining in specimens of 40 normal prostate tissues(NP)and 38 BPH tissues. 方法采用斑點(diǎn)雜交技術(shù)和免疫組化染色測定40例正常前列腺(NP)、38例前列腺增生癥(BPH)組織中b-FGF的表達情況。
- Cell culture in vitro,ABC ELISA,RNA dot blot and atom absorption spectrum analysis were used to study biological effects of low concentration of iron citrate (Fe cit) on the expression of transferrin receptor (TfR) in healthy human peripheral lymphocytes. 為了研究鐵缺乏的生物學(xué)作用,采用體外細胞培養、ABC-ELISA法、RNA斑點(diǎn)雜交和原子吸收光譜分析等技術(shù)方法,觀(guān)察了低濃度檸檬酸鐵(Fe-cit)對健康人外周血淋巴細胞轉鐵蛋白受體(TfR)及其基因表達的影響。
- In Q group the gene expressions of DNA polymerase beta, EGFR and c-myc detected by RNA dot blot array were 6.5 folds, 7 folds and 2.2 folds down-regulated respectively, and the wtp53 was 2.5 folds up. Q組的DNAPolp,EGFR及e一mye的RNA斑點(diǎn)TLe掃描數值分別比對照組下調了6.;5倍,7倍,2
- We measured c fos gene mRNA levels in VSMC with Dig DNA label probe of c fos gene by RNA dot blot; 采用RNA斑點(diǎn)雜交技術(shù),應用地高辛標記的c?fos基因探針檢測培養的兔動(dòng)脈平滑肌細胞中c?fosmRNA水平。
- Detection of Laminin mRNA in Human Primary Liver Cancer Tissue by in situ Hybridization and RNA Dot Blot Hybridization 用原位雜交和RNA斑點(diǎn)雜交法檢測人原發(fā)性肝癌層粘蛋白mRNA
- Theexpression of Eotaxin and CCR3 mRNA in guinea lung tissue was detectedby in situ hybridization (ISH), Northern blot and RNA dot blot; 采用原位分子雜交、Northern雜交、點(diǎn)雜交等方法觀(guān)察肺組織中Eotaxin及其受體CCR3mRNA的表達;
- The gene expressions of DNA polymerase beta, EGFR and c-myc which detected by RNA dot blot array in Br group were 5 folds, 6.5 folds and 2.5 folds down-regulated respectively, and the expression of wtp53 was 2.5 folds up-regulated; RNA斑點(diǎn)印跡顯示:與對照組相比:Br組的DNAPolp,EGFR及c一myc的RNA斑點(diǎn)的TLC掃描數值分別下調了5倍,6.;5倍,2
- The cDNA probe hybridized with BVDV RNA, HCV RNA and yeast tRNA by the dot blot method The BVDV RNA and HCV RNA were positive and yeast tRNA was negative. 瓊脂糖凝膠電泳結果說(shuō)明它與BVDV RNA相似。 該cDNA的探針用斑點(diǎn)雜交方法與BVDV RNA,HCV RNA和酵母tRNA雜交,BVDV RNA和HCVRNA顯陽(yáng)性反應,酵母tRNA顯陰性反應,結果說(shuō)明合成的cDNA為BVDV RNA的cDNA。
- Effect of labeling was detected by dot blot hybridization. 點(diǎn)雜交方法檢測探針標記效果。
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶鏈反應(PCR)結合反向斑點(diǎn)雜交(RDB)技術(shù)。
- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot雜交技術(shù)定量研究3組胃粘膜ET?1、NOSmRNA表達。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis進(jìn)行整合,經(jīng)G418篩選得到25個(gè)高拷貝轉化子,經(jīng)DNA斑點(diǎn)試驗和DNA測序證明外源基因E2穩定地整合到P.;Pastoris染色體中。
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特異性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的:探討斑點(diǎn)雜交法檢測結核分枝桿菌的臨床應用價(jià)值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑點(diǎn)雜交證實(shí)我們制備的探針是敏感而可靠的。
- The objective was to explore the molecular mechanism of electroacupuncture by RNA dot hybriditation in comparison with electroacupuncture and formaline stimulation induced the expression of c fos mRNA. 目的:從分子水平探討電針鎮痛的機理,方法:采用RNA 斑點(diǎn)雜交技術(shù)比較電針與福爾馬林刺激時(shí)大鼠腦內c?fos mRNA 表達的改變,并觀(guān)察嗎啡對c?fos mRNA 表達的影響。
- Though RNA dot hybridization is negative, OP J18-1400 has a conservative domain of seven base pairs DNA sequence: 5-TTCCCTC-3, which is a homologous recombination hotspot domain in atp6 gene. 雖然OPJ18-1400對應的RNA斑點(diǎn)雜交呈陰性,但其DNA序列中存在一個(gè)七堿 基5-TTCCCTC-3的保守系列,它是atp6基因中同源重組熱點(diǎn)區,其促使線(xiàn)粒體基因重組形成嵌