您要查找的是不是：
- PCR產(chǎn)物克隆測序Sequence analysis with clone of PCR products
- 對Sry-PCR產(chǎn)物克隆測序,得到185bp的Sry基因部分核苷酸序列。The partial nucleotide sequence(185 bp)of the Roe deer Sry gene was determined by cloning the PCR product.
- 方法 :用聚合酶鏈反應 (PCR)擴增HPV58L1完整編碼區基因 ,將PCR擴增產(chǎn)物克隆至 pUC1 9質(zhì)粒中并測序。Methods:The full length L1 coden region of HPV58 was amplified by PCR,and cloned into pUC19,sequenced.
- PCR產(chǎn)物直接測序Sequence analysis with PCR products
- 通過(guò)BP反應將包含有attB接頭的PCR產(chǎn)物克隆到含有attP的donor載體上以產(chǎn)生Entry克隆,通過(guò)LR反應將已經(jīng)重組入Entry載體的DREB1A基因再克隆到pH2GW7雙元載體。By the BP recombination reaction,the PCR product containing attB was transfered to an attP-containing donor vector to create an entry clone. Finally,DREB1A gene was shutted into pH2GW7 vector by LR recombination reaction.
- 克隆測序cloning and sequencing
- 利用PCR技術(shù)從豬細小病毒的RFDNA模板中擴增了含有VP2 全基因的 2 0kb的基因片段 ,將PCR產(chǎn)物克隆至 pMD18 T載體 ,利用雙脫氧末端終止法測定了VP2 全基因的核苷酸序列。The completed VP 2 gene was amplified from PPV RFDNA by PCR method. Then the products were cloned into pMD18-T vector and the sequence was determined.
- 克隆測序技術(shù)在小鼠心肌炎實(shí)驗中的應用Application of the Colone and DNA Sequence Test in Myocarditis Mice Model
- coliO_(157):H_7EDL933株的rfbE基因和fliC基因,設計了兩對引物,分別對兩個(gè)O_(157):H_7菌株的rfbE基因和一個(gè)菌株的fliC基因進(jìn)行PCR,并將PCR產(chǎn)物克隆于pMD18-T載體質(zhì)粒。coli O_(157):H_7 , two primers were designed according to the sequence of rfbE gene and fliC gene of E. coli O_(157):H_7 EDL933 strain. The part fragment of rfbE gene of two E.
- 埃及伊蚊抗凝血因子X(jué)a基因片段的克隆測序Part Gene Sequence of Factor Xa-directed Anticoagulant from the Vector Mosquito Aedes aegypti in China
- 可直接克隆PCR產(chǎn)物的克隆載體的構建Construction of A New Vector for Direct Cloning of PCR Products
- PCR測序PCR sequencing
- 產(chǎn)物測序結果The results of sequencing of PRRSV PCR amplified products from Inner Mongolia sequenced
- 方法采用PCR法從結核桿菌H37Rv株擴增Ag85A基因,將PCR擴增產(chǎn)物克隆于2型腺相關(guān)病毒(AAV-2)表達質(zhì)粒pSNAV中,構建重組質(zhì)粒pSNAV-Ag85A;Methods Ag85A gene was amplified by polymerase chain reaction (PCR) from the genome of Mycobacterium tuberculosis H37Rv strain.
- 麥洼牦牛肥胖基因部分片段的克隆測序研究Cloning, Sequencing Research on Partial Fragment of Obese Gene of Bos grunniens
- PCR測序法PCR sequencing
- 利用RT-PCR方法擴增了肉雞HSP70 mRNA,將其擴增產(chǎn)物克隆到PGEM-T Easy載體和原核表達載體pET-28a(+)上,進(jìn)一步轉化至大腸桿菌BL-21中,用IPTG誘導表達重組肉雞HSP70。The mRNA of broiler HSP70 gene was amplified by RT-PCR and the production was cloned into the PGEM-T Easy vector and the expression vector pET-28a (+) . The recombinant expression vector was transformed into E. coli BL-21 and induced by IPTG to express.
- PCR測序(法)PCR sequencing
- 新疆褐牛weaver位點(diǎn)部分序列的克隆測序及分析Partial Cloning and Analysing of Weaver Gene in Xinjiang Brown Cattle
