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- P32基因P32 gene
- 結構蛋白P32是CPV的特異蛋白,包含病毒粒子的主要抗原決定簇,并且P32基因具有很高的保守性,對CPV具有診斷意義。The CPV structural protein P32 was specific to CPV and has a major antigenic determinant, sequence of P32 gene is also conserved to CPV genome.
- 結果表明:GTPV P32基因開(kāi)放閱讀框(ORF)全長(cháng)969bp,編碼323個(gè)氨基酸; 綿羊痘病毒(Sheep pox virus,SPPV)P32基因ORF全長(cháng)972bp,編碼324個(gè)氨基酸;The results of sequencing show that GTPV P32 gene was 969bp and encode 323 amino acids, Sheep pox virus(SPPV) P32 gene was 972bp and encode 324 amino acids, FGD-CPV P32 gene was 969bp and encode 323 amino acids.
- 以PCR方法擴增了切去跨膜結構的P32基因片段,構建了表達部分P32基因的大腸桿菌表達載體pPRO-TRUNCP32,轉化大腸桿菌BL21(DE3),經(jīng)誘導表達出特異的31kDa融合蛋白,蛋白以包涵體形式存在。The smaller truncated P32 fragment without the transmembrane region of P32 was amplified by PCR, and the E. coli expression vector pPRP-TRUNCP32 was constructed. A fusion protein nearly 31kDa was expressed by IPTG-induced BL21(DE3).
- P32蛋白P32 protein
- p32啟動(dòng)子p32 promoter
- 基因代代相傳。Genes are transmitted from one generation to another.
- P32表面蛋白P3 2 surface protein
- 眼P32示蹤器研究P32 tracer study of eye
- 基因外顯率penetrance of gene
- 熒光假單胞菌P32培養基的優(yōu)選STUDY ON THE MEDIUM FOR CULTIVATION OE FLUORESCENT PSEUDOMONRDS STRAIN P32
- 分離的基因型a segregant genotype.
- 基因的區分繁殖differential perpetuation
- Ｃ基因C gene
- Ｄ基因D gene
- Ｊ基因J gene
- Ｖ基因V gene
- Ｉｒ基因Ir gene
- 免疫遺傳學(xué)免疫學(xué)中研究免疫反應的細胞和基因基礎的分支The branch of immunology that deals with the molecular and genetic bases of the immune response.
- ＭＨＣ基因MHC gene
