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- ORF2基因片段ORF2 gene fragment
- ORF2基因ORF2 gene
- 乙肝病毒表面抗原(HBsAg)、戊肝病毒ORF2基因片段NE_2在番茄中的表達及免疫活性Expression and Immunoactivity of HBsAg (hepatitis B Virus Surface Antigen) and HEV-NE2 (hepatitis E Virus ORF2 Gene) in Transgenic Tomato
- 含CpG基序的PCV-2的ORF2基因真核表達載體的構建Construction of Eukaryotic Vector Bearing ORF2 of PCV-2 and Containing CpG Motifs
- 片段segment
- 幽門(mén)螺桿菌對甲硝唑耐藥相關(guān)基因片段的篩選及克隆分析Screening for metronidazole-resistance associated gene fragments of Helicobacter pylori by suppression subtractive hybridization
- 用脂質(zhì)體介導法將pEGFPTTV2質(zhì)粒DNA轉染Cos7細胞,通過(guò)RT-PCR分析,證實(shí)細胞中存在ORF2基因的轉錄產(chǎn)物。The pEGFPTTV2 was transfected into Cos7 cell line using LipoFectamine 2000, and the transcription products of TTV ORF2 gene in the transfected cells were confirmed by RT-PCR.
- M基因片段M gene segment
- 方法通過(guò)巢式PCR,從一份蘭州地區無(wú)償獻血員血清中擴增TTV病毒的ORF2基因,并克隆到pGEM-T載體中,進(jìn)行核苷酸序列分析。Methods The ORF2 gene of TTV was amplified from the serum of a blood donor in Lanzhou region by nest PCR and inserted into vector pGEM-T then,after identification by DNA sequencing,subcloned to expression vector pET22b(+).
- 白血病部分未知基因片段與公共生物信息資源的比較與分析Analysis of some leukemia unknown genes by comparison to public bioinformation resource
- ORF2片段ORF2
- 將兩基因片段分別克隆入pMD-18T載體中,提取克隆質(zhì)粒;The two fragment was fusioned and inserted into pMD-18T victor.
- 假肥大型肌營(yíng)養不良癥cDNA基因片段的表達及其抗血清制備Expression of Duchenne muscular dystrophy cDNA fragment in E.coli and preparation of its antiserum
- 參照GenBank發(fā)表的PCV2BF株基因序列,設計合成一對引物,對PCV2BF株ORF2基因進(jìn)行PCR擴增,產(chǎn)物經(jīng)瓊脂糖凝膠電泳分析,呈現一條約720 bp的特異條帶,將其回收后克隆到pGEM T-Easy載體。A pair of primers was designed according to the PCV2 sequence in Genbank (AF381175). The ORF2 gene, a fragment of 720 bp, was amplified by polymerase chain reaction (PCR), then cloned into pGEM-T Easy vector and identified by BamHl and Xhol digestion.
- ORF2-7基因ORF2- 7 gene
- rDNA基因片段16S rDNA gene frgement
- ORF2截短基因ORF 2 truncated gene
- 特異基因片段unique gene fragments
- 結合上述毒力因子分布情況,根據SS2的cps2J、mrp、orf2基因(virulent-associate sequence orf2)設計合成三對引物,建立三重PCR檢測方法,并對PCR擴增產(chǎn)物進(jìn)行測序、酶切、特異性及敏感性實(shí)驗。The positive detection rates of cps2/gdh+/epf+/mrp+/sly+/fbps+/orf2+ were 96%25 (24/25) and only one virulent SS2 isolate was cps2/gdh+/epf-/mrp+/sly+/fbps+/orf2+.
- 豬鏈球菌2型胞外蛋白因子部分基因片段的克隆表達及小鼠的免疫保護性試驗Cloning and Expression of the Fragment of Epf Gene of Streptococcus Suis Type 2 and the Immune Protection Test in Mice