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- Using genome blast, MAGE-H1/Apr-1 was mapped to chromosome Xp11.22 with the open reading frame (ORF) locating in one exon. 進(jìn)一步在人基因組數據庫利用Blast比較的方法將MAGE-H1/Apr-1定位在Xp11.;22。
- Here, we describe the screening of 10 patients who presented with 46, XY sex reversal for mutations in open reading frame (ORF) of SRY gene. 通過(guò)篩查10位中國46,XY女性性反轉病人SRY基因的開(kāi)放閱讀框區域,探尋新的突變類(lèi)型。
- According to the data of TMV MP in the GeneBank, design gene specific primer, amplified of the whole open reading frame (ORF). 根據GeneBank中TMV MP的序列設計基因特異引物,擴增出運動(dòng)蛋白基因的整個(gè)開(kāi)放閱讀框(ORF)。
- An open reading frame (ORF) is a portion of a gene's sequence that contains a sequence of bases, uninterrupted by stop sequences, that could potentially encode a protein. 開(kāi)放閱讀框是基因序列的一部分,包含一段可以編碼蛋白的堿基序列,不能被終止子打斷。
- Each open reading frame (ORF) is knocked out using a PCR-based gene deletion strategy that takes advantage of the high degree of homologous recombination in yeast. 每個(gè)開(kāi)放讀碼框使用基于PCR基因刪除策略,使用在酵母中的高度同源重組優(yōu)勢。
- An open reading frame (ORF) is a portion of a gene's sequence that contai a sequence of bases, uninterrupted by stop sequences, that could potentially encode a protein. 開(kāi)放閱讀框是基因序列的一部分,包含一段可以編碼蛋白的堿基序列,不能被終止子打斷。
- A synthetic peptide derived from the C terminal sequence of the open reading frame(ORF)j5R was recognized by antisera from pigs recovered from infection with different African swine fever virus(ASFV)strains. 根據非洲豬瘟病毒MalawiLIL20/1株j5R閱讀框C端抗原決定簇序列制備的合成肽能被不同毒株感染康復豬的免疫血清識別。
- Based on the position weight matrix(PWM) and length distribution of open reading frame(ORF),a simple method for predicting translation initiation sites is presented. It can identify TIS from upstream AUGs easily. 結合位置權重矩陣(PWM;position weight matrix)和開(kāi)放閱讀框架(ORF;open reading frame)的長(cháng)度分布特征建立了簡(jiǎn)單的方法識別翻譯起始位點(diǎn);此方法能很好地區分上游AUG和TIS.
- The full-length cDNA of the OsBIRF1 is 1837 bp with a predicted 1191bp open reading frame (ORF), which predicts to encode a 396 amino acid protein containing all conserved domains of the RING finger protein. OsBIRF1基因全長(cháng)cDNA為1873bp,包含一個(gè)1191bp的ORF,編碼一個(gè)含396個(gè)氨基酸的蛋白,并含有RING指蛋白所有的特征性保守結構域。
- The growth hormone cDNA gene (cgh) of grassfish (Ctenopharyngodon idellus) was obtained by RT-PCR, the sequence analysis showed that the ORF (open reading frame) of cgh gene, encoding 210 amino acid residences, was 633 nucleotides. 通過(guò)RT-PCR獲得草魚(yú)(Ctenopharyngodon idellus)生長(cháng)激素cDNA基因(cgh);序列研究表明;cgh基因的ORF為633nt;編碼210aa.
- AmphiANT, was identified from the gut cDNA library of amphioxus B. becheri tisingtauense, consists of 1289 bps and contains an 897 bps open reading frame (ORF) corresponding to a deduced protein of 298 amino acids. AmphiANT全長(cháng)1289 bps,含有一個(gè)897bps的開(kāi)放閱讀框,編碼一個(gè)298個(gè)氨基酸組成的蛋白質(zhì)。
- The SJCWL06 gene is 861 bp in whole length, has an open reading frame (ORF) of 327 bp encoding 109 amino acids with a signal peptide, an ABC-ATPase domain, several phosphorylation sites and the highest similarity of SMC3 protein of Apis mellifera (84%). 該基因長(cháng)861bp,有一個(gè)完整的327bp的開(kāi)放讀框,編碼109個(gè)氨基酸,其編碼蛋白有一個(gè)信號肽,多個(gè)磷酸化位點(diǎn)和一個(gè)ABC-三磷酸腺苷酶的結構域,與蜜蜂(Apis mel-lifera)SMC3編碼的mRNA基因序列同源性最高(84%25)。
- The ZFP28 cDNA sequence is 4104bp and contains a 2076-bp open reading frame (ORF) that encodes an 868 amino acid protein with an N-terminal signal peptide, two KRAB domains and 14 C-terminal C2H2 zinc finger motifs. ZFP28長(cháng)4104個(gè)堿基,含有一個(gè)長(cháng)為2076個(gè)堿基的開(kāi)放閱讀框,編碼一個(gè)長(cháng)為868個(gè)氨基酸的蛋白質(zhì),它包含一個(gè)N-端信號肽,兩個(gè)KRAB結構域和14個(gè)C-端C2H2型鋅指結構。
- The results were summarized as follows: A sequence encoding mannanase, which containing a 1233 bp open reading frame (ORF), was predicted according to the oligonucleotides homologous from LeMan1. 結果如下: 參考番茄中編碼甘露聚糖酶的基因序列,通過(guò)電子克隆的方法預測出一條編碼大豆甘露聚糖酶的核苷酸序列,設計特異引物,采用RT-PCR方法擴增出一條1233 bp的cDNA,命名為GmMan1。
- A full length of NCED cDNA, namely SgNCED1, was cloned and sequenced.It was 2241-bp long and contained an open reading frame (ORF) of 1809-bp which encodes a peptide of 602 amino acids. 1、 柱花草SgNCED1基因的克隆 根據已報道的三種豆科植物的NCED基因同源序列設計簡(jiǎn)并引物,用RT-PCR結合RACE以及嵌套PCR的方法,從水分脅迫的柱花草葉片中克隆了SgNCED1基因,其cDNA全長(cháng)由2241 bp組成,其開(kāi)放閱讀框架為1809 bp,編碼602個(gè)氨基酸。
- CHS gene was cloned from cDNA and CHS open reading frame sequence was obtained. 獲得了CHS基因ORF(Open reading frame)的cDNA序列,全長(cháng)為1299bp。
- Using the Index of Coincidence to identify Open Reading Frames. 使用偶合指數方法來(lái)定出開(kāi)放閱讀框架。
- overlapping open reading frame(overlapping ORF) 重疊開(kāi)放閱讀框
- The Prl cDNA is 1550 bp and contains a 1140 bp open reading frame that is predicted to be a protein of 380 amino acids. Pr1蛋白酶基因cDNA序列長(cháng)度為1550bp;含有一個(gè)1140bp的開(kāi)放閱讀框;編碼380個(gè)氨基酸、分子量為39120、pI=8.;06的蛋白酶前體;
- There is another protein with a different open reading frame from core protein within the HCV C gene. 除了核蛋白外,在丙型肝炎病毒核基因內,尚有一蛋白產(chǎn)生。