A full length of NCED cDNA, namely SgNCED1, was cloned and sequenced.It was 2241-bp long and contained an open reading frame (ORF) of 1809-bp which encodes a peptide of 602 amino acids.
英
美
- 1、 柱花草SgNCED1基因的克隆 根據已報道的三種豆科植物的NCED基因同源序列設計簡(jiǎn)并引物,用RT-PCR結合RACE以及嵌套PCR的方法,從水分脅迫的柱花草葉片中克隆了SgNCED1基因,其cDNA全長(cháng)由2241 bp組成,其開(kāi)放閱讀框架為1809 bp,編碼602個(gè)氨基酸。