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- 10 Zhang JZ,Xu XM.Ma WF,et a1.A rapid reverse dot blot assay [張基增.;徐湘民
- Keywords Prevotella nigrescens;human hemoglobin;iron acquisition;dot blot assay; 黑色普氏菌;人血紅蛋白;鐵攝取;斑點(diǎn)印跡法;
- Effect of labeling was detected by dot blot hybridization. 點(diǎn)雜交方法檢測探針標記效果。
- The results from enzyme-linked assay and dot blot experiment show that an enrichment pool was obtained after four rounds selection, which can specifically be used to recognize ricin. 酶聯(lián)免疫和斑點(diǎn)雜交實(shí)驗結果表明，僅經(jīng)4輪篩選即可獲得特異識別蓖麻毒素蛋白的寡核苷酸適配子。
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶鏈反應(PCR)結合反向斑點(diǎn)雜交(RDB)技術(shù)。
- The expression of fascin was tested by Western blot assay in 26 pairs of ESCC and its cancer-adjacent normal tissues. 在癌旁正常組織中fascin幾乎不表達或表達量很低，而在食管癌組織中fascin表達水平明顯增高。
- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot雜交技術(shù)定量研究3組胃粘膜ET?1、NOSmRNA表達。
- Western?blot assay demonstrates that McAb 4G4,2H4 and FA4 belong to different lipopolysaccharide (LPS) fraction of Aeromonas hydrophila. Western blot結果表明,單抗4G4、2H4、FA4所針對的抗原是菌體脂多糖(LPS),且它們所識別的LPS抗原表位不同。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis進(jìn)行整合，經(jīng)G418篩選得到25個(gè)高拷貝轉化子，經(jīng)DNA斑點(diǎn)試驗和DNA測序證明外源基因E2穩定地整合到P.;Pastoris染色體中。
- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 應用完整細胞原位斑點(diǎn)印跡雜交技術(shù)檢測c-myc、野生型p53(wtp53)、p16和EGFR的基因表達;
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特異性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的：探討斑點(diǎn)雜交法檢測結核分枝桿菌的臨床應用價(jià)值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑點(diǎn)雜交證實(shí)我們制備的探針是敏感而可靠的。
- We have demonstrated the constitutive polypeptides (PP) of Dane parti-cles employing SDS-PAGE Western Blot assay, investigated the antibody res-ponse ability to those PP in the HBV infected subjects in comparison withother HBV serum markers. 采用PAGE-Western Blot技術(shù)對血源性Dane顆粒的多肽組份進(jìn)行了分析。 并結合HBV感染的其它血清學(xué)指標,初步探討了HBV感染者對這些多肽的抗體應答情況及意義。
- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑點(diǎn)雜交法和 Southen 印跡法對腦瘤中 erbB、sis、myc 和 fos 這四種癌基因的擴增和重排進(jìn)行了研究。
- A total of 195 sera from France and Ivory Coast were tested. A good correlation was obtained, all 76 sera with HIV-l antibody by Western blot assay were also positive by PA test and ELISA. 共檢測了195份來(lái)自法國和非洲象牙海岸的血清,凡是蛋白印跡法陽(yáng)性的血清,明膠顆粒凝試驗都是陽(yáng)性。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑點(diǎn)雜交技術(shù)鑒定分枝桿菌菌種的靈敏度為92.;49%25;特異度為100%25。
- Methods: Expression of COX-2 mRNA and its protein in the patients with CML was detected by reverse transcript polymerase chain reaction (RT-PCR) and western blot assay(westernblot). 方法:通過(guò)逆轉錄聚合酶鏈反應(RT-PCR)和蛋白免疫印跡(Westernblot)分別檢測63例慢性粒細胞白血病(CML)患者血細胞中COX-2的mRNA和蛋白的表達;
- After random labeling with DIG, RNA dot blot was proceed to test the expression of this gene under different growth condition. 用地高辛隨機引物標記試劑盒對該片段進(jìn)行標記后,對不同水分和硫營(yíng)養條件下提取的小麥根系總RNA 進(jìn)行斑點(diǎn)雜交。
