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- H9N2亞型AIV基因組序列分析及用反向遺傳技術(shù)產(chǎn)生多個(gè)H9N2和H5重組流感病毒Genomic Characterization of an Avian Influenza Virus of H9N2 Subtype and Generation of H9N2 Reassortants and Attenuated H5 Recombinants by Reverse Genetics
- 將H9N2亞型AIV增殖,經(jīng)差速離心和蔗糖密度梯度離心提純后,滅活,免疫SPF雞,制備高免血清并提純免疫球蛋白G(IgG)。 以此制備疫苗免疫家兔,制備高免血清并提純其中的IgG。High titer serum and immunoglobulin G (IgG) were prepared from specific pathogen free (SPF) chickens immunized with inactivated AIV subtype H9N2. Rabbits were immunized with this chicken anti-H9N2-AIV IgG and high titer serum were collected.
- H9N2亞型AIVH9N2 subtype avian influenza virus
- 因此,我們構建了單表達H5N1亞型AIV血凝素(HA)基因的重組病毒、雙表達H5N1亞型AIV的HA和神經(jīng)氨酸酶(NA)基因的重組病毒及雙表達H5N1和H9N2亞型AIV的血凝素基因(HA)的重組病毒。The purpose of this study isdevelope better recombinant FPV vaccines against H5 and/or H9 subtype AI, in terms ofthe ability to resist the interference of maternal antibodies(MAbs). We constructedrecombinant viruses expressing HA gene of H5 or H9 subtype and coexpressing HA-NAgenes of H5N1 subtype ATV or two HA genes of H5 and H9 subtype AIVs with twodifferent FPV insertion vectors and two different wild type FPVs.
- 以A/African Starling/983/79(H7N1)毒株為模板，人工合成了H7亞型AIV的HA全基因序列，并將其克隆于pMD18-T載體。H7N1 AIV HA gene was synthesized from A/African starling/983/79(H7N7) cDNA template.
- H9N2亞型H9N2 subtype
- H9N2亞型禽流感AIV H9N2
- H9亞型AIVmildly pathogenic avian influenza virus
- H5N1亞型AIVH5N1 subtype avian influenza virus
- H9N2亞型流感病毒H9N2 influenza virus
- H9N2亞型禽流感病毒H9N2 subtype avian influenza virus
- 抗原特異性試驗證明,重組HA1蛋白與雞H5、H9亞型AIV抗血清不發(fā)生交叉反應。An antigen specific test showed that the recombinant did not have any cross-reaction with chicken H5 and H9 anti-sera.
- 禽流感病毒H9N2亞型Avian Influenza Virus H9N2
- H9N2亞型禽流感病毒TJ1株H9N2 subtype Avian influenza virus TJ1 strain
- 甲3亞型Subtype A3
- ζ亞型ζ isoform
- 分子亞型Subtype
- 亞型分析Subtype analysis
- 亞型鑒定Subtype identification
- 亞型學(xué)名Subtype scientific name
