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- Keywords heroin addict;T lymphocyte;flow cytometer（FCM）;immune function.; 海洛因成癮;T細胞亞群;流式細胞術(shù);免疫功能;
- Flow cytometer (FCM) was used to observe the effect of SFPS on cycle and apoptosis of tumor cell. 采用流式細胞儀觀(guān)察羊棲菜多糖對腫瘤細胞周期及細胞凋亡的影響;
- Methods:T lymphocyte subsets of peripheral blood in 50 heroin addicts and 50 healthy subjects were measured with flow cytometer(FCM). 方法:用流式細胞術(shù)檢測50例海洛因成癮者(成癮者)和50例正常人(對照組)外周血T細胞亞群的數目。
- Result Through determining apoptosis rate and cell cycle by flow cytometer (FCM), three kinds of methods can all make cellular apoptosis models. 結果通過(guò)流式細胞儀對細胞凋亡率、細胞周期的檢測,證實(shí)了3種方法均可造成細胞凋亡模型。
- Methods: Flow cytometer(FCM) were used to defect the immunoflourescent stained vWF in pulmonary artery endothelial cells (PAEC) of pig and aortic endothelial cells(AEC) of rats. 方法 :利用免疫組化技術(shù)對體外培養的豬肺動(dòng)脈內皮細胞 (PAEC)和大鼠主動(dòng)脈內皮細胞 (AEC)中vWF進(jìn)行免疫熒光標記 ,通過(guò)流式細胞儀對vWF的細胞陽(yáng)性率和熒光強度進(jìn)行定性、定量分析 ,觀(guān)察低氧和低溫所引起的vWF變化。
- Methods In vivo， the changes of myocardial mechanics were measured by invasive method， and the myocardial cytoskeletal proteins were analyzed by flow cytometer (FCM). 方法在體實(shí)驗，測定大鼠嚴重燒傷后(24小時(shí)內)心肌力學(xué)的變化，同時(shí)采用流式細胞術(shù)定量分析心肌細胞骨架蛋白的動(dòng)態(tài)變化。
- Motheds: Apoptosis of bone marrow cells were measured using methods of DNA content and Annexin V by flow cytometer (FCM) in 48 acute leukemia, 14 MDS, and 16 non-leukemia and non-MDS anemia patients, and the two methods were compared. 方法:采用流式細胞術(shù)DNA含量和(或)膜聯(lián)蛋白(Annexin V)法檢測了48例急性白血病(AL)、14例MDS及16例非白血病、非MDS貧血患者骨髓細胞的凋亡,并對上述兩種凋亡的檢測方法進(jìn)行相關(guān)性比較。
- Methods The CD44,CD54 of peripheral blood was labelled by whole blood immunofluorescence direct-labelled technigue and detected by flow cytometer (FCM) in 25 patients with latent glomerulonephritis. 方法采用流式細胞全血免疫熒光直標術(shù)對25例隱匿型腎小球腎炎患者外周血進(jìn)行CD44,CD54標記后,上流式細胞儀檢測。
- The Application and Development of Flow Cytometer in Infectious Disease Stu-Dy. 流式細胞儀在傳染病研究中的應用與發(fā)展。
- Cell cycle and apoptosis rate was assessed by flow cytometer measurement. 用流式細胞儀檢測A549細胞在使用吉非替尼后不同時(shí)間間隔的細胞周期和凋亡率的變化。
- Cell viability was determined by MTT assay, cell cycle and apoptosis rate were analyzed by flow cytometer. 利用MTT法測定細胞成活率,流式細胞儀測試樣品的細胞周期與凋亡率。
- Methods The FIM score and T-cell were evaluated by flow cytometer in 11 pts with ALS pre-and post-treatment. 方法對11例ALS病人治療前后FIM評分及總T細胞進(jìn)行流式細胞檢測。
- The application of flow cytometer in reticulocyte analysis is introduced at last. 最后,介紹流式細胞儀在網(wǎng)織紅細胞計數方面的應用。
- The expression of Faa and Fas ligand (FasL) was examined by immunofluorescent staining and flow cytometer analysis. 采用流式細胞儀檢測單核細胞 FaS和 Fas Ligand（FasL）的表達；
- Methods Subsets of blood T lymphocyte in 93 patients with SARS were detected by flow cytometer. 方法采用流式細胞儀對93例臨床確診的SARS患者、50例獲得性免疫缺陷綜合征(AIDS)患者及64例健康體檢者外周血T淋巴細胞亞群進(jìn)行檢測。
- Methods Test the activity of CD69 and level of IL-2 by cell flow cytometer after colon cancer operation in 33 cases. 方法應用流式細胞儀檢測33例結腸癌患者手術(shù)前后外周血中T淋巴細胞CD69的表達百分率及IL-2的表達水平,并與對照組進(jìn)行比較分析。
- Methods Cell cycle distribution, cyclins and P27 expression were determined by flow cytometer. 方法用流式細胞技術(shù)測定細胞周期、細胞周期素和P27蛋白表達量。
- After labeled by tyrosine hydroxylase, the differentiated dopaminergic neuron proportion was detected by a flow cytometer. 酪氨酸羥化酶染色標記后通過(guò)流式細胞儀檢測分化的多巴胺能神經(jīng)元比率。
- The CD44V6 and CD49 concentrations in the tumor cells were measured by a flow cytometer(Cell Sorting System)on the day 14 of the treatment. 實(shí)驗d14以流式細胞儀檢測各組小鼠移植瘤組織中瘤細胞CD44V6及CD49的表達。
- Method Flow cytometer and hematology analyzer were used to detect the expression of lymphocytes and its subsets in 38 patients of SARS. 方法采用多色流式細胞術(shù)結合血液分析儀測定38例SARS患者外周血淋巴細胞及其亞群的表達情況。
