Methods: Flow cytometer(FCM) were used to defect the immunoflourescent stained vWF in pulmonary artery endothelial cells (PAEC) of pig and aortic endothelial cells(AEC) of rats.
英
美
- 方法 :利用免疫組化技術(shù)對體外培養的豬肺動(dòng)脈內皮細胞 (PAEC)和大鼠主動(dòng)脈內皮細胞 (AEC)中vWF進(jìn)行免疫熒光標記 ,通過(guò)流式細胞儀對vWF的細胞陽(yáng)性率和熒光強度進(jìn)行定性、定量分析 ,觀(guān)察低氧和低溫所引起的vWF變化。
