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- Rearrangement and amplificarion of erbB,sis,myc and fos in brain tumors are studied with DNA dot blot and Southern blot analysis. 本文用 DNA 斑點(diǎn)雜交法和 Southen 印跡法對腦瘤中 erbB、sis、myc 和 fos 這四種癌基因的擴增和重排進(jìn)行了研究。
- DNA dot blot analysis showed that 73. 4% of resistant plants contained GNA and hpt genes. Southern blot analysis confirmed the integration of GNA gene in the genome of transgenic rice. DNA點(diǎn)雜交檢測表明73.;4%25的抗性植株同時(shí)含有GNA基因和hpt基因;Southern雜交分析進(jìn)一步證實(shí)了GNA基因在轉基因水稻基因組中的整合
- Apply the PCRDIG probe to quickly detect the SRY gene by DNA dot blotting. PCR-DIG探針斑點(diǎn)雜交法快速檢測SRY基因
- The results showed that, positive signals of DNA Dot blots for V-sis, c-erb B-2 , V-abl, H-ras, K-ras and c-myc are found. Among them the signals of c-sis and c-erb B-2 are stronger than the others. 結果發(fā)現,V-sis、c-erbB-2、V-abl、H-ras、K-ras和c-myc質(zhì)粒DNA的斑點(diǎn)出現陽(yáng)性雜交信號,其中V-sis、c-erbB-2的斑點(diǎn)信號最強。
- It was proved that the E2 genes were integrated stably into chromosome of P.Pastoris by Dot blot and DNA sequencing. Pastotis進(jìn)行整合，經(jīng)G418篩選得到25個(gè)高拷貝轉化子，經(jīng)DNA斑點(diǎn)試驗和DNA測序證明外源基因E2穩定地整合到P.;Pastoris染色體中。
- Effect of labeling was detected by dot blot hybridization. 點(diǎn)雜交方法檢測探針標記效果。
- Regenerated plants with kanamynic resistantance were obtained. PCR , PCR-Southern blot analysis , southern dot blot analysis of lettuce DNA confirmed that adw gene had been integrated into the plant genome. 細胞核載體PB-adw、PBG-adw均采用農桿菌介導法將adw導入萵苣，細胞核轉化獲得了生長(cháng)良好的抗性萵苣植株，經(jīng)PCR、PCR-Southern、southern斑點(diǎn)雜交分析證實(shí)，adw基因已整合到萵苣基因組中。
- In Q group the gene expressions of DNA polymerase beta, EGFR and c-myc detected by RNA dot blot array were 6.5 folds, 7 folds and 2.2 folds down-regulated respectively, and the wtp53 was 2.5 folds up. Q組的DNAPolp，EGFR及e一mye的RNA斑點(diǎn)TLe掃描數值分別比對照組下調了6.;5倍，7倍，2
- The results of reverse spot hybridization for both duck hepatitis B virus DNA (DHBV DNA) and special DNA polymerase were compared with those by dot blot for DHBV DNA and electromicroscopy for DHBV particles. 本文將逆向斑點(diǎn)雜交同時(shí)檢測鴨乙型肝炎病毒DNA(DHBV DNA)及其特異DNA多聚酶(DNAp)的結果,與斑點(diǎn)雜交檢測DHBV DNA和電鏡檢測DHBV顆粒的結果作了比較。
- Dot blot hybridization with the two probes on Nitrocellulose Membranes showed the positive results for PPV DNA from different resources and PUP recombinant plasmid,but negative for the nucleic acid samples obtained from a series of control virus. 分別對不同來(lái)源的豬細小病毒DNA及PUP重組質(zhì)粒于硝酸纖維素膜上打點(diǎn)雜交，免疫呈色后均為陽(yáng)性反應，而對照的豬瘟病毒、乙型腦炎病毒、偽狂犬病毒、PK－15細胞的核酸均為陰性反應。
- Dot blot hybridization with the two probes showed the positive result for PPV DNA,but negative for the nucleic acid samples obtained from Hog cholera virus,Pseudorabies virus,Japanese B Encephalitis virus and PK 15 cells. 對豬細小病毒DNA進(jìn)行斑點(diǎn)雜交，兩種探針均為陽(yáng)性，而對照的豬瘟病毒、豬偽狂犬病毒、乙型腦炎病毒及PK－15細胞的核酸均為陰性。
- Methods Polymerase chain reaction(PCR) connected with reverse dot blot(RDB) were performed. 方法采用多聚酶鏈反應(PCR)結合反向斑點(diǎn)雜交(RDB)技術(shù)。
- ET?1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique. 采用Dotblot雜交技術(shù)定量研究3組胃粘膜ET?1、NOSmRNA表達。
- Besides, the gene expression of c-myc, wtp53, p16 and EGFR was detected by RNA dot blot. 應用完整細胞原位斑點(diǎn)印跡雜交技術(shù)檢測c-myc、野生型p53(wtp53)、p16和EGFR的基因表達;
- Denatured-reduced forms of MBP-fusion proteins in immunoblotting, dot blot and ELISA.General. 特異性 Monoclonal Anti-GFP recognizes wild type; recombinant; and enhanced form of GFP.
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的：探討斑點(diǎn)雜交法檢測結核分枝桿菌的臨床應用價(jià)值。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑點(diǎn)雜交證實(shí)我們制備的探針是敏感而可靠的。
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑點(diǎn)雜交技術(shù)鑒定分枝桿菌菌種的靈敏度為92.;49%25;特異度為100%25。
- After random labeling with DIG, RNA dot blot was proceed to test the expression of this gene under different growth condition. 用地高辛隨機引物標記試劑盒對該片段進(jìn)行標記后,對不同水分和硫營(yíng)養條件下提取的小麥根系總RNA 進(jìn)行斑點(diǎn)雜交。
