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- (二)實(shí)驗方法:每位入選患者抽取外周靜脈血1.0ml,以TaKaRa全血DNA提取試劑盒提取DNA,以exon3、exon4及exon7的目標片斷為模板進(jìn)行PCR擴增,然后對擴增產(chǎn)物進(jìn)行基因測序。Methods: Took 1.0ml venous blood from every recruited patient, extrac-ted DNA with TaKaRa whole blood Genome kit, PCR amplified the objective fragments of exon3, exon4 and exon7, genotyped all the amplified fragments.
- DNA提取試劑盒DNA extraction kit
- 胰腺組織RNA含量豐富 ,極易降解RNA ,操作速度、RNA提取試劑盒的質(zhì)量和防止RNA污染的綜合措施是成功制備高質(zhì)量RNA的關(guān)鍵The pancreas in rat is abound in Rnases, which makes RNA in pancreas stay in high risk of degradation. The important factors for gaining high quanlity of RNA from pancreas are processing quickness of preparation RNA and the quality of reagent.
- 動(dòng)物組織總蛋白提取試劑T-PER Tissue Protein Extraction Reagent
- 落葉松-楊柵銹菌DNA提取新方法研究A renewed DNA extraction method for molecular study of Melampsora larici-populina
- 從農用鉀肥中提取試劑級氯化鉀的工藝條件研究Extraction of Analytical Reagent Potassium Chloride from Agricultural Potassium Manure
- 福爾馬林固定石蠟包埋組織DNA提取方法對pcr的影響Extraction of DNA from formalin fixed and paraffin embedded tissues and its effect on PCR
- 全國人體重要寄生蟲(chóng)病現狀調查中囊蟲(chóng)病試劑盒批間差異的比較Comparison of difference among three groups of cysticercosis kit in the national survey on the important parasitic diseases
- DNA提取DNA extraction
- 診斷試劑盒diagnostic reagent kit
- 總DNA提取DNA extraction
- 兩種方法均以硼砂為緩沖液,分別用SDS、和CTAB為主要提取試劑,可以提取出高質(zhì)量的木本植物總RNA;The main reagent of extraction buffer is borax, and the main extraction reagent is SDS and CTAB respectively in the two extraction methods.
- 食品安全和反興奮劑領(lǐng)域常用藥物快速檢測試劑盒的開(kāi)發(fā)Development of ELISA Kats and gold test strips in food safety and performance-enhancing drugs
- DNA提取量Amount of extracted DNA
- T3試劑盒Trilute
- 胚乳DNA提取endosperm DNA extraction
- 鈣試劑盒Calcium
- DNA提取方法DNA extraction
- PCR試劑盒PCR kit
- 微量DNA提取mini-exaction DNA