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- 重組NP蛋白recombinant nucleoprotein(NP)
- 自H5N1陽(yáng)性病料中提取總RNA,反轉錄后采用高可信度DNA聚合酶,經(jīng)PCR擴增NP基因,采用Invitrogen定向表達系統進(jìn)行克隆表達,純化獲得N末端攜帶多聚組氨酸標簽的重組NP蛋白,分子質(zhì)量約66.0ku。Total RNA was extracted from positive clinical sample of H5N1 subtype of avian influenza virus, NP gene was amplified by reverse transcriptase-polymerase chain reaction using high proofreading polymerase (PyobestTM DNA Polymerase), and expressed using Invitrogen championTM pET directional TOPO expression system. Recombinant protein containing polyhistidine (6xHis) tag in N-terminal was about 66.0 ku in size, had been obtained and purified.
- 重組reform
- 資產(chǎn)重組reorganized assets; assets reorganization
- NP蛋白NP protein
- 企業(yè)重組reshuffle of the enterprises
- 禽流感病毒NP蛋白avian influenza virus nucleoprotein
- 重組N蛋白recombinant N protein
- 重組E蛋白Equine West nile virus antibody
- 重組M蛋白recombinant M protein
- 重組A蛋白recombinant protein A
- 重組S蛋白S proteins
- 重組Cap蛋白recombinant Cap protein
- E2重組蛋白E2 recombinant protein
- 重組GP5蛋白recombinant GP5 protein
- 重組P1蛋白recombinant P1 protein
- 重組P25蛋白recombinant P25 protein
- 重組VP2蛋白recombinant VP2 protein
- 重組VP7蛋白recombinant VP7 protein
- 重組gD蛋白recombinant gD protein
