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- 重組質(zhì)粒pGEX-Csn轉化E.Recombinant plasmid pGEX-Csn was transformed into E.
- 結果構建了重組質(zhì)粒pGEX-4T-CTB,CTB基因片段分子量約為376bp;Finally,the recombinant plasmid pGEX-4T-CTB was successfully constructed with a CTB gene fragment of 376 bps.
- 將重組質(zhì)粒pGEX一SAGI轉化大腸桿菌BL21一CodonPlus(DE3)一RP菌株中，在IPTG誘導下表達;The recombinant plasmid of pGEX-SAGl was transformed to a bacterium BL21-Codon Plus(DE3)-RP and the recombinant protein was expressed under the inducement of IPTG.
- 用IPTG誘導重組質(zhì)粒pGEX-2T/htL-PGDS表達的最佳濃度為1 mmol/L,最佳時(shí)間為4 h.The optimal concentration of IPTG, which can induce the recombina nt pGEX -2T/ht L-PGDS to express fusion GST/htL-PGDS protein , is 1mmol/L, and the o ptimal induction period is 4h.
- 將報告基因綠色熒光蛋白基因(gfpmut3a)分別置于recA和uvrA啟動(dòng)子調控下,構建成質(zhì)粒pRecAgfp和pUvrAgfp,并轉化E.Plasmids pRecAgfp and pUvrAgfp were constructed after DNA damage-inducible promoters of recA and uvrA from Escherichia coli were fused to the reporter gene gfpmut3a operon.
- 方法用PCR方法擴增ctxB目的基因片段,克隆至pQE30-napA質(zhì)粒的napA基因上游,構建含雙基因的表達質(zhì)粒pQE30-napA-ctxB(pQE30-nctB),經(jīng)測序分析確認后轉化E.Methods The ctxB gene was amplified by PCR and cloned into plasmid pQE30-napA. The recombinant plasmid was identified by sequencing, and then transformed into E.
- 重組質(zhì)粒dna recombinant plasmid
- 旋毛蟲(chóng)ES抗原特異性蛋白兩個(gè)結構基因的序列分析及重組質(zhì)粒的構建SEQUENCING OF TWO STRUCTURAL GENES ENCODING SPECIFIC PROTEINS IN ES ANTIGEN FROM TRICHINELLA SPIRALIS AND CONSTRUCTION OF RECOMBINANT PLASMIDS
- 方法設計引物,采用PCR法分別擴增UreB表位多肽編碼基因Uepi和LTB編碼基因,重疊延伸PCR法將兩段基因拼接,T-A克隆后,構建融合基因表達質(zhì)粒pET-22b(+)-Uepi-LTB,經(jīng)酶切鑒定后轉化E.Methods Amplify the genes encoding UreB epitope polypeptide and LTB by PCR respectively and link by overlap extension PCR. After T-A cloning,the linked gene Uepi-LTB was cloned into prokaryotic expression vector pET-22b(+),and the constructed recombinant plasmid pET-22b(+)-Uepi-LTB was transformed to E.
- 本研究對含有BDV p2 4重組質(zhì)粒PGEX 3X的大腸桿菌表達系統進(jìn)行了優(yōu)化表達BDV p2 4蛋白 ,在IPTG 2mmol/L、3h表達量最大 ,同時(shí)用BDV p2 4單克隆抗體證實(shí)了其特異性。A method for BDV p24 specific antibody detection was established based on experiments by carrying out optimization of expression of BDV p24 protein expressed by recombinant plasmid PGEX 3X containing BDV p24 in E. coli . The expression was the highest at 2mmol/L of IPTG in 3h.
- 前列腺特異性膜抗原啟動(dòng)子調控的重組質(zhì)粒的構建及表達The Construction of Recombinant Plasmid with Prostate-specific Membrane Antigen Promoter Controlling Reporter Gene Expression
- coliBL21中，利用轉化子在RBB一木聚糖平板上形成的透明圈，分別篩選到含有重組表達質(zhì)粒pET一BCxE和重組表達質(zhì)粒pGEX一BcXE的陽(yáng)性表達子E.coli BL21. Two positive recombinants, E. colt BCE5 containing pET-BCXE vector and E. coliBCE1 harboring pGEX-BCXE vector, were obtained, which forming zones of clearing on RBB-xylan plates.
- 重組質(zhì)粒構建construction of recombinant plasmid
- 水稻葉綠體DNA克隆片段的分析和rbcL基因在重組質(zhì)粒上的定位Characterization of A Cloned DNA Fragment of Rice Chloroplast and Location rbcL Gene in the Recombinant Plasmid
- TIMP-1重組質(zhì)粒TIMP-1 recombinant plasmid
- 氧化硫硫桿菌重組質(zhì)粒pSDT125的構建及其在大腸桿菌之間的轉移CONSTRUCTION OF THIOBACILLUS THIOOXIDANS RECOMBINANT PLASMID pSDT125 AND ITS MOBILIZATION AMONG E. COLI STRAINS
- 為了實(shí)現蛋白內含肽(Intein)介導的重組環(huán)狀胸腺五肽結構類(lèi)似物[cyclo-(Cys-Arg-Lys-Asp-Val-Tyr-),cTP]的高效制備,設計并合成編碼6個(gè)氨基酸的cTP基因,克隆到表達載體pTWIN1,重組表達質(zhì)粒pTW-cTp轉化E.In order to obtain the recombinant Tp-5 cyclic analogue [cyclo-(Cys-Arg-Lys-Asp-Val-Tyr-),cTP]efficiently by intein-mediated single column purification, a gene encoding 6-amino acids peptide was designed, synthesized and cloned into Escherichia coli expression vector pTWIN1. The recombinant vector pTW-cTP was transferred into E.
- 重組質(zhì)粒pRSETDNAThe recombinant plasmid pRSET DNA
- pSH-CUP重組質(zhì)粒構建constructing the recombinant plasmid pSH-CUP
- 微囊化人類(lèi)心房肽cDNA重組質(zhì)粒轉染細胞對實(shí)驗性高血壓大鼠腎組織學(xué)改變的影響Effects of Encapsulated Plasmid Recombining with hANP cDNA Transfected Cells on Morphological and Histological Characteristic of Experimental Hypertensive Rats
