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- 載體pVAX1pVAX1 vector
- 將HeB02株F基因插入真核表達載體pVAX1中,構建了真核表達質(zhì)粒pSV-F,通過(guò)脂質(zhì)體轉染COS-7細胞,SDS-PAGE分析可見(jiàn)表達的特異蛋白條帶;The expression plasmid pSV-F was constructed by inserting the F gene into the pVAX1 vector, and transfected into the cultured COS 7 cell line via liposomes.
- 載體carrier
- 方法用PCR法從HCMV基因組中釣取gB680和pp65m蛋白基因,分別亞克隆入pMD18-T載體中,經(jīng)酶切鑒定并測序后,構建真核表達載體pVAX1/gB680+pp65m,以ELISA法檢測其在COS-7細胞中的瞬時(shí)表達。Methods Amplify gB680 and pp65m genes from genome of HCMV by PCR and subclone to vector pMD18-T respectively. After identification of the constructed recombinant plasmids by restriction analysis and sequencing,clone gB680 and pp65m genes into plasmid pVAX1.Transfect COS-7 cells with the constructed eukaryotic expression vector pVAX1/gB680+pp65m,and identify the expressed product by ELISA.
- 將SL7207(pVAX1-F)以109CFU/只劑量口服免疫1日齡雛雞,免疫保護試驗結果顯示,SL7207(pVAX1-F)免疫組對雞具有良好的保護率(77.27%),與空白對照組和SL7207(pVAX1)空載體組之間存在顯著(zhù)性差異(P<0.05)。The chickens immunized with SL7207(pVAX1-F) could be protected with the protective rate of 77.27%25,significantly higher than those with SL7207(pVAX1)(29.42%25) or PBS(12.5%25)(P<0.05).
- 承載體supporting body
- 表達載體expression vector
- 桿菌載體bacillus carrier
- 硅膠載體silica-gel carrier
- 白色載體white support
- 氧化鋁載體alumina supporter
- 薄殼載體pellicular support
- 信息載體semantide
- 氨基酸轉運載體amino acid carrier
- 鹵載體halogen carrier
- 氯載體chlorine carrier
- 白色硅藻載體Chamelife
- 報導用載體announcement media
- 病毒載體virus vector
- 反載體hold-back carrier
