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- 然后將VP1基因分段克隆至原核表達載體pGEX-4T-1，經(jīng)酶切及PCR鑒定，證明成功構建了重組表達載體pGEX-A、pGEX-B、pGEX-C。Based on the analysis results, three partition of VP1 gene of CAV were cloned and inserted into the bacterial plasmids pGEX-4T-1. The recombinant plasmids containing VP1 partiton gene of CAV were identified by restriction enzyme analysis and PCR method.
- 載體pGEXvector pGEX
- 載體carrier
- 結果構建了重組質(zhì)粒pGEX-4T-CTB,CTB基因片段分子量約為376bp;Finally,the recombinant plasmid pGEX-4T-CTB was successfully constructed with a CTB gene fragment of 376 bps.
- PCR拼接法擴增殼聚糖酶基因并克隆入pGEM-Teasy載體進(jìn)行序列分析,進(jìn)一步亞克隆入表達載體pGEX-3X。coli favorites. The Csn gene was cloned into plasmid pGEM-Teasy and verified by DNA sequence analysis. Thereafter, Csn gene was subcloned into a fusion-protein expressing vector pGEX-3X.
- 將煙夜蛾的UBE基因克隆到原核表達載體pGEX-4T-2上,經(jīng)IPTG誘導,SDS-PAGE電泳檢測出約40kD的融合蛋白。The fragment containing UBE gene was inserted into pGEX-4T-2 expressive vector, and induced by IPTG. Molecular weight of ubiquitin fusion protein was about 40 kD by checking with SDS polyacrylamide gel electrophoresis.
- 承載體supporting body
- 表達載體expression vector
- 以克隆的激活蛋白基因為目的基因，構建了原核表達載體pGEX-XF-1，轉化到大腸桿菌BL21和ER2566中，通過(guò)IPTG誘導，得到高效體外表達。Cloned activator protein gene was inserted into pGEX-5X-l to construct the recombinant prokaryotic expression vector, pGEX-XF-1. Then the vector was transformed into E. coli BL21 and ER2566. And high expression in vitro induced by IPTG was obtained and analyzed on SDS-PAGE gel.
- 桿菌載體bacillus carrier
- 葡萄球菌A蛋白（SPA）和鏈球菌G蛋白（SPG）的IgG結合區編碼基因融合后，克隆到大腸桿菌表達載體PGEX，SPA/SPG融合蛋白（SPAG）得到高效表達。The encoding genes of IgG binding domains of staphyloccal protein A(SPA)nd streptococcal protein G (SPG)were cloned and expressed as fusion protein using pGEX vector of E. coli.
- 硅膠載體silica-gel carrier
- 白色載體white support
- 氧化鋁載體alumina supporter
- 薄殼載體pellicular support
- 信息載體semantide
- 氨基酸轉運載體amino acid carrier
- 鹵載體halogen carrier
- 氯載體chlorine carrier
- 白色硅藻載體Chamelife
