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- 轉座載體pUT／mini-Tn5transposon vector pUT/mini-Tn5
- 方法 :將 HGV NS3基因片段定向克隆至轉座載體 p Fast Bac HTa,轉化 DH10 Bac感受態(tài)細胞 ,37℃振蕩培養 4h使發(fā)生轉座 ,用抗生素平皿篩選重組 bacmid。Methods: Recombinant plasmid pFHTNS3 was constructed by inserting HGV NS3 gene into transposing vector pFastBac HTa. pFHTNS3 plasmid was further transformed into DH10Bac competent cells.
- 轉to convey
- 座to sit
- 載體carrier
- 白羊座Aries
- 轉帳transfer money from|into an account
- 摩羯座Capricornus
- Tn5轉座子Tn5 transposon
- 處女座virgo
- 轉寄forward
- 軸承座shaft block
- 轉矩torque
- 轉晴fair
- 座席pew
- 轉置transposition
- 座套seating
- 轉出roll out
- Mini-Tn5轉座子Mini- Tn5 transposon
- 鞍座bicycle saddle
