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- 質(zhì)粒pEGFP-N1pEGFP-N1
- 方法以質(zhì)粒pEGFP-N1為模板擴增EGFP基因,EcoR I和Hind III雙酶切EGFP基因序列和pET28a載體。Methods Using plasmid as template,full-length EGFP gene was amplified. The amplified EGFP gene and pET28a vector were cleaved by EcoR I and Hind III,then ligated after purifying.
- 將其克隆入pEGFP-H1/siRNA,構建表達EGFP及TLR4-shRNA的真核表達質(zhì)粒pEGFP-H1/TLR4-siRNA。Then an oligo nuclear hairpin sequence targeting TLR4 gene was designed by the internet tool siRNA Wizard and then inserted into the plasmid pEGFP-H1/siRNA so as to form the plasmid pEGFP-H1/TLR4-siRNA.
- 方法:將殼聚糖納米粒包裹的含報告基因的質(zhì)粒pEGFP-C1分別轉染前列腺癌細胞PC-3和22Rv1,并觀(guān)察EGFP的表達情況。Methods:Prostate cancer cells PC-3 and 22Rvl were transfect- ed with plasmid pEGFP-C1 coated with chitosan nanoparticles and the expression of EGFP was observed.
- 觀(guān)察差異表達SJCHGC蛋白重組真核表達質(zhì)粒pEGFP-C1/SJCHGC在COS-7細胞中的表達和亞細胞定位,并分析其表達產(chǎn)物的抗原性.To observe the in vitro expression and subcellular localization of recombinant eukaryotic expression plasmid pEGFP-C1/ SJCHGC in COS-7 cells and analyze the antigenicity of the products expressed.
- 質(zhì)粒plasmid
- 目的構建抑制大鼠心肌細胞k ir2.1基因的真核表達質(zhì)粒pEGFP 6-k ir2.1,觀(guān)察對大鼠心肌細胞k ir2.1信使RNA(mRNA)、蛋白表達情況及搏動(dòng)頻率的影響。Objective To construct the expression short hairpin RNA(shRNA) targeting gene kir2.1 in rat myocardial cells,named pEGFP6-kir2.1,and to observe the effects on the expression of messenger RNA(mRNA) and protein of gene kir2.1 as well as the changes of myocardial beating rates.
- pEGFP-N1質(zhì)粒Plasmid pEGFP-N1
- 重組質(zhì)粒dna recombinant plasmid
- Ｒ質(zhì)粒R plasmid
- Ｒｉ質(zhì)粒Ri-plasmid
- Ｔｉ質(zhì)粒Ti-plasmid
- 胞質(zhì)粒albuminous granules
- 不接合質(zhì)粒nonconjugation plasmid
- ti 質(zhì)粒ti plasmid
- 嵌合質(zhì)粒chimeric plasmid
- 質(zhì)粒工程plasmid engineering
- 質(zhì)粒排除plasmid elimination
- 質(zhì)粒轉導plasmid transduction
- 自主質(zhì)粒autonomous plasmid
