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- 利用PCR方法以人胎肝cDNA文庫為模板,克隆重組人肝細胞生長(cháng)因子(rhHGF)全長(cháng)cDNA,經(jīng)序列分析后,插入pPIC9K中,構建多拷貝串聯(lián)rhHGF基因酵母分泌型表達載體pPIC9K-rhHGF,轉化酵母宿主菌Pichia Pastoris GS115。The cDNA encoding rhHGF was amplified by PCR using cDNA library of fetal liver tissues as template,and was insert-ed,after being confirmed by DNA sequence analysis, into the vector pPIC9K to form a recombinant Pichia pastoris secretory expression vector pPIC9K-rhHGF ,which contain many copies of rhHGF gene .
- 構詞因素
- pPIC9K表達載體Pichia pastoris
- 表達to voice (an opinion)
- 表達載體pPIC9KExpression vector pPIC9K
- 構建了真核表達載體pPic9k-DCN，并將酶切線(xiàn)性化的重組載體轉入酵母菌HIS~-/GS115后，篩選出了抗高濃度(4mg/ml)G418的陽(yáng)性克隆，并用PCR法進(jìn)行了鑒定。We have made up an eukaryotic (Pichia pastoris) expressive vector (pPic9k-DCN). After the linear recombinant vector was introduced into HIS~-/GS115 cells,we have selected positice clones against G418(4mg/ml)and confirmed by PCR.
- 將ScFvA1-Etag基因，GLS融合基因重組進(jìn)大腸桿菌-酵母穿梭質(zhì)粒pPIC9K, 分別構建含有ScFvA1-Etag基因，GLS融合基因的表達載體pPIC9K-ScFvA1-Etag，pPIC9K-GLS。The ScFvA1-Etag and the glucose-linker-ScFvA1 (GLS) fusion gene were cloned into the vector pPIC9K and expressed in methylotrophic yeast Pichia pastoris GS115, respectively, under the control of the AOX1 promoter.
- HBV全基因核心蛋白變異株穩定表達載體的構建及其抗原表達CONSTRUCTION OF STABLE EXPRESSION VECTORS WITH HBV GENOME CAPSID PROTEIN MUTANTS AND THEIR ANTIGENIC EXPRESSION
- 芳香烴受體及其核轉運蛋白融合表達載體的構建與序列鑒定Construction and sequence analyzing of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator
- 共表達載體coexpression vector
- DNA表達載體DNA expression vecotor
- pET表達載體pET expression vector
- 表達載體法siRNA expression vectors
- 原核表達載體prokaryotic expression vector
- 表達載體構建Expression vector construction
- 基因表達載體gene expression
- RNAi表達載體RNAi expression vector
- 雙元表達載體binary expression vector
- 酵母表達載體yeast expression vector
