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- 首先通過(guò)PCR的方法，將編碼大鼠DAF四個(gè)SCR的基因片段亞克隆到Trx融合原核表達載體pET32a(+)。Firstly, I subcloned the DNA fragment that encode the four consensus repeat of DAF into the prokaryotic expression vector pET32a(+) which could add a Trx tag on the N-terminal of the target protein.
- 方法:采用RT-PCR技術(shù)從人肝癌細胞SMMC-7721中擴增出剪切掉線(xiàn)粒體定位信號的人AIF基因片段,并按閱讀框克隆到原核表達載體pET32a(+)中。METHODS: A human AIF gene fragment of 1515 bp(mitochondrial localization sequence was deleted) was amplified from SMMC-7721 cells by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into pET32a(+) vector to construct recombinant plasmid pET32a-AIF.
- 表達載體pET32aexpression vector pET32a
- 方法利用PCR技術(shù)擴增tlh基因,克隆至載體pET32a+并測序。 將測序結果提交國際互聯(lián)網(wǎng)上有關(guān)生物信息網(wǎng)站進(jìn)行生物信息學(xué)分析。Methods The tlh gene amplified by PCR was cloned into the vector pET32a+ and sequenced, followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet.
- HBV全基因核心蛋白變異株穩定表達載體的構建及其抗原表達CONSTRUCTION OF STABLE EXPRESSION VECTORS WITH HBV GENOME CAPSID PROTEIN MUTANTS AND THEIR ANTIGENIC EXPRESSION
- 芳香烴受體及其核轉運蛋白融合表達載體的構建與序列鑒定Construction and sequence analyzing of aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator
- 共表達載體coexpression vector
- DNA表達載體DNA expression vecotor
- pET表達載體pET expression vector
- 表達載體法siRNA expression vectors
- 原核表達載體prokaryotic expression vector
- 表達載體構建Expression vector construction
- 基因表達載體gene expression
- RNAi表達載體RNAi expression vector
- 雙元表達載體binary expression vector
- 酵母表達載體yeast expression vector
- pIL2表達載體pIL2 plasmid
- 質(zhì)粒表達載體plasmid expression vector
- 高效表達載體High-efficient expression vector
