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- 蛋白酶K法proteinase-K pathway
- 針對粘菌標本(孢子)數量少、形態(tài)微小等特點(diǎn),分別采用CTAB法、SDS法、蛋白酶K法4和SDS-蛋白酶K4種方法,提取基因組DNA。Considering the fact that the specimens of myxomycetes are small in amount and minute in form, four methods, CTAB method, SDS method, protease K method and SDS - protease K method, are used to extract genomic DNA.
- 法law
- 無(wú)法unable
- 組織蛋白酶KCathepsin K
- 摘要梯度K法以歐氏距離公式為基礎，應用于電測深找水，它能夠利用實(shí)測數據較準確地確定最佳井位和估計涌水量，并獲得較佳的地質(zhì)效果。Based on Euclidean distance, applied in water detection by electrical sounding, The gradient K method can estimate the location of the well and its flowing yield with measured data, and can obtain satisfactory geologic results.
- 蛋白酶KProteinase K
- 說(shuō)法statement
- 熱硼酸／蛋白酶K法hot borate/Protease K method
- 輸入法input method
- 除法division
- 經(jīng)蛋白酶K消化處理的ECPs,銀染可見(jiàn)脂多糖(LPSs)的典型O 糖側鏈結構,顯示脂多糖是組成粗制ECPs的重要組分之一。When treated with protoase K digestion and stained by silver assay, typical O-specific side chains of liposaccharides could be observed on the electrophoritic gel, which suggested that LPS was one of the most abundant component in the gross ECPs of Aeromonas hydrophlia.
- 分析法analytical method
- 商法commercial law
- 通過(guò)優(yōu)化實(shí)驗,確定了用SAP、Exo I和蛋白酶K聯(lián)合使用對PCR產(chǎn)物進(jìn)行純化,可短時(shí)間、低成本、高效的獲得PCR純化產(chǎn)物;The method combines the SAP, Exo I and proteinase K to purify the PCR product, determined by our optimal experiment, can obtain the purified product with the short time, low cost and high efficiency;
- 賓夕法尼亞Pennsylvania
- 讀法pronunciation
- 法輪功Falun Gong Cult
- 通過(guò)蛋白酶K消化過(guò)程中對于酶的濃度、溫浴時(shí)間的比較試驗,發(fā)現隨著(zhù)采用大幅提高酶濃度、延長(cháng)消化時(shí)間等高強度的蛋白酶消化操作后,DNA的質(zhì)量和產(chǎn)量均得到顯著(zhù)提高。We used three pretreatment methods to remove formalin:gradual dehydration by alcohol,GTE buffer displacement and critical point drying,by comparison the DNA from samples treated by critical point drying is the best in quality and quantity. Intensive proteinase K treatment including increased proteinase K concentration and prolonged digestion time also greatly enhances quality and quantity of DNA.
- 法門(mén)method
