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- 競爭性定量RT-PCRcompetitive quantitative RT-PCR
- 主要包括相對定量RT-PCR(Relative quantitative RT-PCR)、競爭性定量RT-PCR(Competitive quan-titative RT-PCR)、比較定量RT-PCR(Comparative quantitative RT-PCR)和實(shí)時(shí)定量RT-PCR(Real time quantitative RT-PCR)四種。In particular,it is the most sensitive and reproducible method for the detection of low-abundance mRNA,often obtained from limited tissue samples. In fact,this technique is sensitive enough to enable quantitation of RNA from a single cell. There are four kinds of quantitative RT-PCR: relative quantitative RTPCR,competitive quantitative RT-PCR,comparative quantitative RT-PCR and real time quantitative RT-PCR.
- 目的 建立針對 AML- ETO融合基因轉錄本的競爭性定量反向 -聚合酶鏈反應 (RT- PCR)體系 ,并探討其在 t(8;2 1)急性髓細胞性白血病 (acute myeloid leukemia,AML)微小殘留病變跟蹤中的應用價(jià)值。Objective To construct and evaluate the competitive quantitative RT PCR method for detecting the fusion gene of AML1 ETO in the patients of t(8;21)AML(acute myeloid leukemia).
- 定量RT-PCRQuantitative RT-PCR
- 應用競爭性定量PCR(QC-PCR)技術(shù),對石化廢水處理系統中投加的高效芳烴降解菌株Rhodococcus ruber Em1數量變化趨勢及其作用進(jìn)行了分析。A quantitative competitive PCR (QC-PCR) system was developed to quantify the number and analyze the function of the Rhodococcus ruber Em1 strain in a wastewater treatment system in Nanchong oil refinery plant.
- 病毒RNA分子拷貝數和靶基因mRNA水平用實(shí)時(shí)定量RT-PCR確定。The RNA copies of viral vectors and mRNA levels of target genes were determined by quantitative real-time RT-PCR.
- 比較定量RT-PCRcomparative quantitative RT-PCR
- 應用RT-PCR檢測人工感染番鴨體內番鴨呼腸病毒的動(dòng)態(tài)分布Dynamic Distribution of Muscovy duck reovirus in the Challenged Ducks Detected by RT-PCR
- 競爭性PCRcompetitive PCR
- 用定量RT-PCR方法檢測腦梗死半影區內凋亡相關(guān)基因的表達Quantitative RT-PCR in detecting the apoptosis associated gene expression in penumbra area of cerebral infarction in rats
- RT-PCRRT-PCR
- IC-RT-PCRIC-RT-PCR
- 半定量RT-PCRsemi-quantitative RT-PCR
- 常規RT-PCRconventional RT-PCR
- 實(shí)時(shí)定量RT-PCR(Real-time PCR)檢測Evi-1,MDS1/Evi-1表達。The Evi-1 and MDS1/ Evi-1 genes expression were examined by fluorescent Realtime PCR.
- 競爭性RT-PCRcompetitive RT-PCR
- 定量PCRquantitative PCR ( qPCR)
- PCR定量Quantitative polymerase chain reaction (PCR)
- 半定量PCRSend-quantitative PCR
- 實(shí)時(shí)定量PCRReal-time PCR
