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- 人黑色素濃集激素1型受體真核表達載體的構建及穩定轉染CHO細胞系的建立Construction of eukaryotic expressing vector of human melanin-concentrating hormone receptor 1 and establishment of stable transfectant CHO cell line
- 穩定轉染的CHO細胞系stable transfected CHO cell line
- 穩定轉染的HepG2細胞系Stable transfected HepG2 cell lines
- 穩定轉染的SHG-44細胞系stable transfected SHG-44 cell line
- 轉染VMAT2的CHO細胞對DA引起的毒性有顯著(zhù)的抵抗作用。VMAT2 transgenic CHO cells can resist the toxicity of dopamine.
- 幽門(mén)螺桿菌cagA基因穩定轉染胃上皮細胞及其對細胞的作用Effect of stable transfection of gastric epithelial cellls with recombinant of Helicobacter pylori cagA gene
- 篩選出了能高效穩定表達CTLA-4/Ig融合蛋白的CHO細胞;The CHO cells that could steadily express at high level the CTLA-4/Ig fusion protein was selected for cloning.
- 逆轉錄病毒介導的對原代培養成年大鼠成肌細胞基因轉染的研究Study of Gene Transfection Mediated by Retrovirus in Primary Cultured Adult Rat Skeletal Myoblasts
- 轉染transfection
- 高拱壩結構穩定轉異和體形優(yōu)化分析理論和方法Analysis Theory and Method of Stabilization Anomaly and Shape Optimization for High Arch dams
- 腹腔內注射TNF基因轉染的LAK細胞對于腹水型肝癌小鼠的療效Therapeutic Effect of TNF Gene-transfected LAK Cells on the Ascitic Liver Carcinoma-bearing Mice
- 穩定轉導反義增殖細胞核抗原基因對膀胱癌細胞體外增殖活性的調控作用The regulation effects on in vitro proliferation activities of human bladder cancer cells by stable antisense proliferating cell nuclear antigen gene transfer
- GCV和CTL對HSV-TK/B7基因轉染的大鼠乳腺癌細胞殺傷的體外實(shí)驗In vitro cytotoxicities of GCV and CTL to rat mammary cancer cells transfected with HSV-TK/B7 gene
- 為了排除其它粘附分子的作用 ,應用聯(lián)合轉染DR7或 (和 )B7分子基因的CHO細胞系作為人工抗原遞呈細胞 (APC)介導混合淋巴細胞反應 (MLR)。B7 transfected CHO cells were used as artificial antigen presentation cells (APCs) in MLR to exclude the effects of other costimulatory molecules.
- 穩定轉染stable transfection
- 基因轉染的SCsgene-transfection schwann cells
- 胰島素樣生長(cháng)因子1基因轉染對大鼠肌腱細胞生長(cháng)的促進(jìn)作用Insulin-like growth factor 1 mediated gene transfer to rat tendon cells to promote the growth of the tendon cells
- 目的構建人谷胱甘肽硫轉移酶A1(GSTA1)的CHO細胞表達體系。 方法重組質(zhì)粒pDNA3.1-GSTA1用lipofectamineTM2000轉染CHO細胞,用G418篩選細胞的陽(yáng)性克隆,并用PCR、RT-PCR和Western blot進(jìn)行鑒定,測定GSTA1酶活性。Objective To establish the Chinese Hamster Ovary(CHO) expression system for the human glutathione-S- transferase (GST) A1. Methods CHO cells was transfected with recombinant vector pDNA3.1- GSTA1 by lipofectamineTM 2000. The expression of selected CHO-GSTA1 in G418-resistant clones were determined by PCR,RT-PCR and Western blot.
- 子宮內基因轉染的研究進(jìn)展Progress in utero gene transfer
- 全營(yíng)養液促脂質(zhì)體介導質(zhì)粒DNA轉染結腸直腸癌細胞的實(shí)驗研究Experimental study of total nutrient admixture promoting transfection of plasmid DNA mediated with liposomes to colorectal cancer cells
