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- 將此ORF連接入質(zhì)粒pSE-380中，并在大腸桿菌JM109和BL21中表達。The ORF was inserted into expression vector pSE-380, then transformed into E. coli JM109 and E.
- 大腸桿菌JM109Escherichia coli JM109
- 本研究應用PCR技術(shù)從大腸桿菌JM109(E.coli JM109)中擴增出磷酸絲氨酸轉氨酶基因，將其與表達載體pEC 7連接。In this study the SerB gene was obtained from E. coli Jm109 by PCR and inserted into pEC7 and this recombinant plasmids with SerB gene were transformed into Brevibacterium flavum C-l1 (BfC-11 ) by the method of electrotransformation.
- 將構建好的重組質(zhì)粒pKK223.3-RTA和pKK223.3-RTA-YQRL轉化感受態(tài)大腸桿菌JM109，經(jīng)IPTG誘導表達RTA和RTA-YQRL蛋白。Expression and purification of recombinant RTAsThe expression plasmid Pkk223.3-RTA was introduced into E. coli JM 109 by CaCl2-mediated method.
- 應用PCR法從弓形蟲(chóng)QHO株速殖子基因組DNA中擴增ROP2的基因片斷,連接到pMD18-T載體中,轉化大腸桿菌JM109,經(jīng)酶切及PCR鑒定后進(jìn)行測序,并進(jìn)行序列分析.The gene coding for rhoptry protein2(ROP2) from the total DNA of Toxoplasma gondii of QHO strain was cloned and analyzed in the study. The gene fragment encoding ROP2 was amplified by PCR from the total DNA of Toxoplasma gondii of QHO strain. And the gene of ROP2 was subcloned into the plasmid pMD18-T.
- 基因工程菌JM109（pGEX-AZR）JM 109 ( pGEX - AZR)
- 大腸埃希氏菌, 大腸桿菌Escherichia coli
- Coli JM109宿主菌中進(jìn)行表達,實(shí)驗結果表明經(jīng)轉化的E.Coli JM109 host cell. The result shows that E.
- 大腸桿菌含量level of Escherichia coli
- 大腸桿菌PGDH末端缺失突變體的構建及抗反饋抑制效應分析Construction and Characterization of E. Coli PGDH Mutants with Feedback-inhibition Resistance
- 用42℃熱激誘導JM109(pBV220-cit)精氨酸脫亞氨基酶的表達,但是未檢測到酶活。However, the ADI enzyme activity of JM109(pBV220-cit) had not been detected.
- 鴨大腸桿菌pathogenic Escherichia coli from duck
- 薐
- 禽大腸桿菌APEC
- coli JM109/pTrc-rCR; IPTG誘導重組蛋白的表達,SDS-PAGE分析得到重組蛋白表達條帶;The recombinant Escherichia coli JM109 strain harboring the expression plasmid was induced by IPTG, gained the rCR protein band by SDS-PAGE analysis, and the specific activity of the E.
- 大腸桿菌K12E. Coli K12
- 大腸桿菌O85E. coli O85
- F18大腸桿菌Escherichia coli F18
- 大腸桿菌F18Escherichia coli F18
- 兔大腸桿菌rabbit E. coli
