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- 基因芯片的PCR引物設計PCR primers design for gene chip
- 通過(guò)引物設計、PCR、基因克隆、測序,獲得了10種鳶尾屬植物和1個(gè)外類(lèi)群種的ITS序列。ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
- 檢測泌尿生殖道感染常見(jiàn)性病病原體基因芯片的構建Development of a Gene Chip for the Detection of Common Pathogens Causing Urogenital Sexually Transmitted Infections
- 檢測拉米夫定耐藥位點(diǎn)基因芯片的研制及其應用初探The pilot study on development of lamivudine-resistance oligonucleotide microarray and its reliability in clinical application
- 應用簡(jiǎn)并引物RT-PCR擴增鋅指結構域篩選新基因Identification of New EST and Genes with Zinc Finger Motif by RT-PCR Using Degenerate Primers
- "... 我要用利劍一樣的話(huà)刺痛她的心，但決不是真用利劍 ... ""... I will speak daggers to her, but use none ... " (Shakespeare, Hamlet)
- "Harmsworth先生，" 我用微弱的聲音說(shuō)。 "別打斷我的說(shuō)。" 他說(shuō)。"Mr Harmsworth, " I said in a weak voice, "Don't interrupt, " he said.
- 用新設計引物調查武漢漢族Penta D和Penta E基因座遺傳多態(tài)性Genetic polymorphisms of the pentanucleotide repeat loci Penta D and Penta E in Wuhan Han population by re-designed primers
- "Inthefamilyway" 是一種老式的表達方式，意為 "懷孕" 。"In the family way" is an old-fashioned expression meaning "pregnant".
- 烷不溶物heptane insoluble matter
- 如果荷蘭不改變這個(gè)決定，中國同荷蘭的關(guān)系肯定要倒退。If the Netherlands refuses to alter its decision,Sino-Dutch relations will definitely suffer a setback.
- 政府打算建造許多新的旅館,一個(gè)巨大的露天運動(dòng)場(chǎng)和一個(gè)漂亮的新游泳池。The government will be building new hotels, an immense stadium, and a fine new swimming pool.
- 根據參數生成數值柵格要素,它具有指定的范圍大小和數值,并輸入轉換流程。NumericRasterCreator Creates a feature with a raster of the specified size with a numeric value using the parameters supplied, and sends it into the workspace for processing.
- 如果中華人民共和國“竊缺到了我們目前所部署的每種核彈頭的“設計情報”的話(huà)，那么，責任將部分歸咎于克林頓政府目前執行的這些政策。If the PRC has managed to "steal" "design information" on every one of our currently deployed nuclear warheads,then each of these Clinton Administration policies is partially to blame.
- 她的同學(xué)并沒(méi)有對她在衣服上的品位以及精心修飾的指甲留下深刻印象，更糟糕的是，her classmates aren't too impressed by her taste in clothes or obsession with manicures and, to make matters worse
- 根據已克隆R基因普遍具有的NBS結構域的P-loop、Kinase-2a、Kinase-3a、EGF區設計5條RGA簡(jiǎn)并引物，組成5對引物組合。Five degenerated primers were designed according to the P-loop, Kinase-2a, Kinase-r3a, EGF domain of NBS which universally exist in cloned R genes. PCR amplifications were performed with the designed RGA primers and cDNA as templates.
- 只要為了實(shí)現一些明確的目標,如更好的武器或軍事運輸系統,美國政府愿意花錢(qián)研究。The government was willing to pay for research only to meet certain clear goals, such as better weapons or military transport systems.
- 以型特異性引物PCR法檢測16對乙肝疫苗阻斷失敗的母嬰和88例阻斷成功的母親血清樣本中HBV基因型,用PCR產(chǎn)物直接測序法檢測HBV前C/ BCP突變,采用Clustal W 1.8軟件進(jìn)行序列分析。HBV genotypes were determined by type-specific primers PCR method. PreC/BCP mutations were detected by direct sequencing of PCR products,and Clustal W 1.8 software was applied to analyzing the sequences.
- 根據黑曲霉F044脂肪酶N-端氨基酸序列,運用生物信息學(xué)方法,找到與黑曲霉脂肪酶基因同源的候選基因A84689.根據該基因序列,設計引物直接經(jīng)RT-PCR擴增得到黑曲霉脂肪酶全長(cháng)基因anl.From the N-terminal amino acid sequence of the Aspergillus niger F044 lipase,a potential homologous gene A84689 of the anl(the gene encoding the Aspergillus niger lipase) was found by means of bioinformatics. Based on the nucleotide sequence of the A84689,primers were designed to amplify anl.
